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PeproTech RANKL (soluble) Polyclonal Antibody, PeproTech®, Invitrogen™

Catalog No. 500P6350UG
Encompass
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100 μg
50 μg
1 mg
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Catalog No. Quantity
500P6350UG 50 μg
500P63100UG 100 μg
500P631MG 1 mg
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Catalog No. 500P6350UG Supplier PeproTech Supplier No. 500P6350UG

Description

Rabbit Polyclonal Antibody

AA Sequence of recombinant protein: PAMMEGSWLD VAQRGKPEAQ PFAHLTINAA SIPSGSHKVT LSSWYHDRGW AKISNMTLSN GKLRVNQDGF YYLYANICFR HHETSGSVPT DYLQLMVYVV KTSIKIPSSH NLMKGGSTKN WSGNSEFHFY SINVGGFFKL RAGEEISIQV SNPSLLDPDQ DATYFGAFKV QDID. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine sRANK Ligand. Anti-Murine sRANK Ligand-specific antibody was purified by affinity chromatography employing an immobilized Murine sRANK Ligand matrix. Sandwich ELISA: To detect Murine sRANK Ligand by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine sRANKL (500-P63Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine sRANK Ligand. Western Blot: To detect Murine sRANK Ligand by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine sRANK Ligand is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.

Specifications

Antigen RANKL (soluble)
Applications ELISA, Western Blot
Classification Polyclonal
Concentration 0.1-1.0 mg/mL
Conjugate Unconjugated
Formulation PBS with no preservative
Gene TNFSF11
Gene Accession No. O35235
Gene Alias CD254; H-RANK-L; hRANKL2; Ly109l; M-Rank-L; ODF; OPG; OPG ligand; OPGL; OPTB2; Osteoclast differentiation factor; osteoprotegerin ligand; Rankl; rank-l; receptor activator of NF-kappaB ligand; receptor activator of nuclear factor kappa B ligand; receptor activator of nuclear factor kappa-B ligand; RP11-86N24.2; sOdf; srankl; TNF-related activation-induced cytokine; Tnfsf11; TNLG6B; Trance; tumor necrosis factor (ligand) superfamily member 11; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand 6B; tumor necrosis factor ligand superfamily member 11; Tumor necrosis factor ligand superfamily member 11, membrane form; Tumor necrosis factor ligand superfamily member 11, soluble form; tumor necrosis factor superfamily member 11; tumor necrosis factor-related activation-induced cytokine
Gene Symbols TNFSF11
Host Species Rabbit
Immunogen E.coli-derived Recombinant Murine sRANK Ligand.
Purification Method Antigen affinity chromatography
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 21943
Target Species Mouse
Content And Storage -20°C
Product Type Antibody
Form Lyophilized
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