Invitrogen™ Rabbit anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor™ Plus 488

Description

Rabbit anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor™ Plus 488 can detect both Fab2 and Fc region of Mouse IgG. It can also detect all subtypes of Mouse IgG (Mouse IgG1,2a,2b and 3). It has <20% cross reactivity to Human IgG and Rat IgM. It showed minimum to no cross-reactivity to various species immunoglobulins tested. This recombinant antibody has been engineered with point mutations that eliminate binding to Fc receptors, enabling reduction in background and superior signal to noise. Superclonal™ recombinant secondary antibodies are produced using a multifaceted clonal selection process that involves several phenotypic screens to ensure enhanced specificity, animal origin free formulation and lot-to-lot consistency. Alexa Fluor™ Plus dyes have been chemically optimized to provide higher signal to noise ratios and minimized cross-reactivity to help detect low-abundance targets in rare or precious samples. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.