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Rabbit anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Invitrogen™

Catalog No. A27022
Encompass
Catalog No. A27022 Supplier Thermo Fisher Scientific Supplier No. LSA27022
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Description

Rabbit Recombinant Polyclonal Secondary Antibody

The sensitivity and specificity of each lot is confirmed using ELISA. Minimal cross-reactivity with goat, rat, human, bovine, guinea pig, and donkey IgG is observed. Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds – the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody – recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets – a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

Antigen Mouse IgG (H+L)
Applications Immunocytochemistry, Western Blot
Classification Polyclonal
Conjugate Unconjugated
Formulation PBS with 0.09% sodium azide
Host Species Rabbit
Immunogen Recombinant full-length protein
Purification Method Protein A
Regulatory Status RUO
Primary or Secondary Secondary
Target Species Mouse
Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form Liquid
Isotype IgG
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