Invitrogen™ Rabbit anti-Mouse IgG Fc Superclonal™ Secondary Antibody, PE

Rabbit Recombinant Superclonal Secondary Antibody

Rabbit anti-Mouse IgG Fc Superclonal™ Recombinant Secondary Antibody, PE is specific to Fc fragment of Mouse IgG. It can detect all subtypes of Mouse IgG (Mouse IgG1, 2a, 2b and 3). It has less than 15% cross reactivity to Human IgG. Minimal to no cross-reactivity was observed with immunoglobulins from the other species tested. This recombinant antibody has been engineered with point mutations that eliminate binding to Fc receptors, enabling reduction in background and superior signal to noise. Superclonal™ recombinant secondary antibodies are produced using a multifaceted clonal selection process that involves several phenotypic screens to ensure enhanced specificity, animal origin free formulation and lot-to-lot consistency. R-phycoerythrin (R-PE) is an intensely bright phycobiliprotein isolated from red algae that exhibits extremely bright red-orange fluorescence with high quantum yields. In practical applications, the sensitivity of R-PE conjugates is usually 5 to 10 times greater than those of the corresponding fluorescein conjugates. R-PE is a large molecule used for fluorescence-based detection, primarily in flow cytometry, microarray assays, ELISAs, and other applications that require high sensitivity, but not photostability. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.