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Invitrogen™ Qubit™ 4 Fluorometer, with WiFi

Catalog No. Q33238
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Catalog No. Q33238 Supplier Invitrogen™ Supplier No. Q33238
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Description

Qubit™ 4 Flurometer with WiFi is designed to accurately measure DNA, RNA, and protein quantity, and now also RNA integrity and quality. Re-engineered to enable data transfer via WiFi as well as to run the Qubit™ RNA IQ (integrity & quality) assay.

  • Qubit™ 4 Fluorometer and RNA IQ Assay Kit work together to accurately distinguish intact from degraded RNA in just two easy steps
  • For all assays, the concentration or quality of the target molecule in the sample is reported by a fluorescent dye that emits a signal only when bound to the target, which minimizes the effects of contaminants on the result
  • Easy-to-use touch-screen menus make it easy to select and run the assays, with results displayed in just a few seconds

Features:

  • Fast and highly accurate quantitation of DNA, RNA, and protein in less than three seconds per sample
  • Measures intact RNA in less than 5 seconds per sample
  • High levels of accuracy using only 1 to 20 μL of sample, even with very dilute samples
  • On-board Reagent Calculator quickly generates Qubit™ working solution preparation instructions
  • Stores results from up to 1000 samples
  • Large 5.7 in, state-of-the-art, color touch screen for easy workflow navigation
  • Graphical display indicates when samples are in the extended range or out of range
  • Small footprint saves space on your bench
  • Flexibility to export data to using the included WiFi dongle, a USB drive, or directly to your computer via a USB cable
  • Ability to personalize your Qubit™ fluorometer with the assays you run most, add new assays, or even create your own assays with the MyQubit™ software and web tool
  • Language of your choice including English, French, Spanish, Italian, German, simplified Chinese, and Japanese
  • Pre-loaded program to test performance of fluorometer using the Qubit™ Fluorometer Verification Assay Kit

Flurometer can also be used to directly measure the fluorescence of samples using Fluorometer mode.

  • Ion Sphere Particle quality can be evaluated on the Fluorometer using the Ion Sphere Quality Control Kit prior to performing a sequencing run on the Ion PGM sequencer.

Enables more accurate results than with UV absorbance at low concentrations

  • Fluorometer is far more sensitive than UV absorbance, which measures anything absorbing at 260 nm, including DNA, RNA, protein, free nucleotides, or excess salts
  • Dyes in the Qubit™ assay kits fluoresce only when bound to the selected molecule (DNA, RNA or protein) in a sample, preventing repetating work due to inaccurate measurements

Fast, simple, and accurate measurement of RNA quality

  • Qubit™ RNA IQ assay provides a fast, simple method to check whether an RNA sample has degraded
  • Assay utilizes two unique dyes: one that binds to large, intact RNA, and another that selectively binds to small, degraded RNA, together enabling quick assesment of the quality and integrity of an RNA sample.
  • To use, simply add samples to the RNA IQ working solution, then measure on the Fluorometer.

Save samples and bench space

  • Requires samples of only 1 to 20 μL
  • Intended for use at room temperature and occupies only a small amount of bench space
  • Features advanced optics and data analysis algorithms, and comes equipped with a WiFi dongle, a USB thumb drive, and cable for data transfer to Excel software and for software downloads, as well as a universal power supply, four plug adapters, and electromagnetic CE certification
  • Enables maximum flexibility for data export using WiFi, USB drive, or USB cable direct to computer.
  • All data can be saved in the cloud application, including direct transfer from the instrument via WiFi.

Single sample measurements are a breeze

  • User-friendly fluorometer is combined with quick and simple assay procedures
  • Calculations and settings are automatically performed by the instrument
  • Asays are all performed using the same general protocol, which uses a simple mix-and-read format with incubation times of only two minutes for the DNA and RNA assays.
  • 500 L thin-walled PCR tubes are required

Specifications

Sample Volume 1 to 20 μL
Excitation Wavelength 470 nm (Blue LED), 635 nm (Red LED)
For Use With (Equipment) Qubit Fluorometer
Product Line Qubit
Type Fluorometer
For Use With (Application) DNA Quantification, RNA Quantification, Protein Quantification
Shipping Condition Room Temperature
Quantity 1 fluorometer

Frequently Asked Questions (FAQs)

What is the standard warranty of the Qubit 4 Fluorometer, with WiFi?

3 years is the standard warranty period.

I would like to use my Qubit 4 Fluorometer with the Qubit 1X dsDNA BR Assay, but the assay is not listed as an option on the fluorometer. How can I resolve this?

The Qubit 4 Fluorometer needs to be updated. The Qubit 4 Fluorometer can be updated with the USB that was provided with the Qubit. If you do not have the USB, we recommend using an empty 2.0GB that is FAT32 formatted. If the USB is not recognized, leave USB plugged in for a few minutes before running the update. This should give you the assay you need to run the Qubit Assay.

You can find the updates on the Technical Resources for Qubit Fluorometers page.
My Qubit 4 Fluorometer, with Wifi (Cat. No. Q33238) has not installed the files to allow me to use the Qubit ssDNA Assay Kit. Could you provide the files I need?

To access the Qubit ssDNA Assay Kit on the Qubit 4, please try selecting the Oligo option from the home screen, and the ssDNA Assay should appear there. If not, please check if you need to update your instrument firmware to the latest version v2.19, which is available for download from the Technical Resources for Qubit Fluorometers page on thermofisher.com.
For standard/preloaded Qubit assays we do not provide individual .qbt files, but they are included in the firmware itself.
How can I use the Qubit Protein Broad Range Assay (Cat. No. A50668) on the Qubit 4 Fluorometer, with WiFi (Cat. No. Q33238)?

To be able to use the Qubit Protein Broad Range Assay (Cat. No. A50668) on the Qubit 4 Fluorometer (Cat. No Q33238) you would need to install the latest firmware on the fluorometer. Please find the latest Qubit 4 firmware (v2.19) on the following webpage: https://downloads.thermofisher.com/Qubit4/v2.19/Qubit4_update_v2.19.pak Save the firmware update on a USB stick and upload it to the Qubit 4 Fluorometer. Once the firmware update has been installed, you will be able to see the Protein BR assay on the Qubit 4 Fluorometer.

How can I access user settings when using the Qubit 4 Fluorometer, with WiFi (Cat. No. Q33238)?

You have to create a user profile and log in. Please follow the instructions for login below:

  • Turn on the device
  • From the home screen, touch the person icon on the top left corner
  • Touch the “Get Started” button
  • Touch the “Create Profile” button
  • information for username and pin
  • Log in by touching the person icon and entering the user info in the sign in fields
I want to perform a system verification on my Qubit 4 Fluorometer, with WiFi (Cat. No. Q33238). However, under "Settings", the System Verification is inactive. How should I proceed?

Due to cybersecurity regulations, it is mandatory to create a profile on the device before all features become available. Please follow these steps:

  • Click on the user icon in the upper left corner.
  • Click on Get Started. The device will guide you through the steps to create a profile.
  • Select a pin and confirm the creation of the profile.
  • Log in with this new profile and you should now have all the features available.
    • You can also find instructions on how to set up a user profile on the Qubit 4 Fluorometer on page 2 of the release notes.

Which Qubit Fluorometers allow the designing of custom assays?

Custom assays can be designed on Qubit 3 and Qubit 4 Fluorometers. For more information, please see the following link.
I am trying to update the software on my Qubit 4 Fluorometer, with WiFi (Cat. No. Q33238). However, this option appears to be inactive. What should I do?

Due to the security settings of the Qubit 4 Fluorometer, with WiFi (Cat. No. Q33238), you will need to be logged in as a user to access the full settings menu. When not logged in as a user, options such as instrument settings, software update and system verification remain inactive.

You can find instructions on how to set up a user profile on the Qubit 4 Fluorometer on page 2 of the Firmware release notes.
What routine maintenance is recommended for the Qubit 4 Fluorometer, with WIFI (Cat. No. Q33238)?

The Qubit 4 Fluorometer, with WIFI (Cat. No. Q33238), does not require any specific routine maintenance. However, based on customer experience, we recommend the following:

  • From time to time, it may be useful to check the opening of the Qubit for dust particles and dirt. If needed, you can use a lint-free cloth or lenses cleaning paper towels to wipe the inside of the Qubit with some 75%-95% ethanol.

  • If you experience inaccurate measurements, regardless of the kit you use, we recommend testing the light source of the instrument with the Qubit 4 System Verification Assay Kit (Cat. No. Q33238).
I cannot find the Qubit 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits (Q33266) assay file to download to my Qubit instrument. Where can I find the necessary file?

To access the necessary file for the Qubit 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits, update the Qubit 4 firmware using this link. The newest firmware contains the required assay file.
The settings options on my Qubit instrument are greyed out and I am being prompted to sign in. How can I resolve this?

Our Qubit 4 and Qubit Flex instruments will now require users to sign in to access certain menu options (i.e., instrument settings, software update, and system verification). This is necessary to adhere to cybersecurity legal standards that were put in place in 2020. Only when signed in, are all functions of the device are accessible.

Instructions for login:
- Turn on the device
- From the home screen, touch the person icon on the top left corner
- Touch the "Get Started" button
- Touch the "Create Profile" button
- Enter information for username and pin
- Log in by touching the person icon and entering the user info in the sign in fields
I am trying to perform a measurement on my Qubit 4 Fluorometer (Cat. No. Q33238), but all the application buttons are grayed out and I am unable to select them as a result. How do I resolve this issue?

To resolve this issue, create a user profile prior to measuring samples. If the problem persists, please contact technical support for further assistance at techsupport@thermofisher.com.

Which is the best DNA or RNA quantification instrument?

All of the DNA instruments offered by Thermo Fisher Scientific are of excellent quality. To find the right instrument to quantify DNA or RNA in your lab, explore the detailed comparison of UV-Vis spectrophotometers and fluorometers. Things like sensitivity, throughput and budget may be initial considerations when selecting a DNA quantification instrument. You may also consider whether you need target specificity, sample purity information, RNA quality information, or broad dynamic range.
What is the difference between UV and fluorescence DNA quantification? Which DNA quantification technology should I choose?

UV and fluorescence technologies work differently to quantify DNA. UV quantification relies on the intrinsic absorptivity of DNA and RNA molecules, while fluorescence quantification uses dyes that specifically bind to your molecule of choice. With UV technology, quantification isn't as sensitive, but it has a broader dynamic range and also gives data about sample purity‒plus it is faster because there is no reagent prep. With fluorescence technology you get higher sensitivity and molecule-specific data, but it has lower dynamic range and reagent prep is required. The technology you need will depend on what features are important for your lab and experimentation.
How do I connect my Qubit 4 Fluorometer, with WiFi (Cat. No. Q33238) to the Cloud?

To connect the Qubit 4 Fluorometer, with WiFi (Cat. No. Q33238) to the Thermo Fisher Cloud, you need to have an active Thermo Fisher Connect account. You can create an account here (https://www.thermofisher.com/us/en/home/digital-science/thermo-fisher-connect.html) and then try again.

If you have an active Thermo Fisher Connect account and your instrument is connected to the internet, you can try these approaches:
1. Confirm that the date, time, and time zone are correct.
2. Try a workaround in which you sign into the local account and then sign into Thermo Fisher Connect within the export data screen.
The steps are:
    a. Go to a data set, click the checkbox next to it
    b. Export
    c. CSV or PDF
    d. Select "Cloud" and follow the on-screen instructions.

I am setting up my Qubit 4.0 Fluorometer, with WiFi (Cat. No. Q33238), and I only have access to "About Instrument" in settings. How do I gain access to the full menu in settings?

Due to the security settings of the Qubit 4 Fluorometer, with WiFi (No. Q33238), you will need to be logged in as a user to access the full settings menu.

You can find instructions on how to set up a user profile on the Qubit 4 Fluorometer on page 2 of the Qubit 4 Fluorometer Firmware v2.18 Release Notes.

Can you provide an IQOQ for the Qubit Fluorometer, with wifi (Cat. No. Q33238)?

We do not have an IQ/OQ qualification service for the Qubit Fluorometer.

For Research Use Only. Not for use in diagnostic procedures.