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MilliporeSigma™ ProteoExtract™ Native Cytoskeleton Enrichment

Catalog No. 1710210
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32 Assays/Kit
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Catalog No. Quantity
1710210 32 Assays/Kit
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Catalog No. 1710210 Supplier MilliporeSigma™ Supplier No. 1710210
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Description

Includes

  • One bottle containing 1.9mL of 10x Cellular Extraction Buffer
  • One bottle containing 3.5mL of 20x Cytoskeleton Wash Buffer
  • One bottle containing 9mL of ready to use Nuclear Extraction Buffer
  • One vial containing 100μL of 1000x Protease Inhibitor Cocktail solution
  • One vial containing 150μL of 1000x Sodium Orthovanadate solution
  • One vial containing 50μL of 200x donkey anti-mouse FITC conjugate
  • One bottle containing 12.5mL of 10x Blocking/Permeabilization Buffer
  • One vial containing 15μg lyophilized TRITC-Conjugated Phalloidin
  • One vial containing 100μL of 200x DAPI
  • One vial containing 100μL of 100x anti-Vinculin mouse monoclonal IgG1 antibody
  • One vial containing 16μL of 500x anti-GAPDH mouse monoclonal IgG1 antibody

Requires

  • Pipettors and tips capable of accurately measuring 1-1000μL
  • Graduated serological pipettes
  • Distilled or deionized water
  • 4% paraformaldehyde solution for fixing cells
  • 1x Dulbecco's Phosphate Buffered Saline (D-PBS)
  • Cover slips
  • Mounting fluid

This method provides means to directly study cytoskeleton-associated pool of proteins in normal and diseased cytoskeletons

  • Provides cytoskeleton purification detergent buffers, sufficient for extraction from ten 100mm culture dishes to retain focal adhesion and actin-associated proteins while removing soluble cytoplasmic and nuclear proteins from the cell.
  • Anti-Vimentin and anti-GAPDH antibodies are provided as markers for cytoskeleton and cytosol, respectively, in western blot analysis
  • This kit will greatly increase the ability to detect and study the low abundance actin-associated proteins which are typically masked in conventional methods of Western blotting analysis of whole cell lysates

Specifications

Content And Storage The kit may be used for up to 4 months after receipt. Avoid repeated freeze/thaw cycles, and aliquot if necessary
For Use With (Application) Immunofluorescence, Immunocytochemistry
Quantity 32 Assays/Kit