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Promega™ Restriction Enzymes - Sgf I Genome Qualified (C) DFS Item

Genome qualified to ensure optimal performance in genomic analysis applications. Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications.

Manufacturer:  Promega™ R7103

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Catalog No. PR-R7103



Description

Description

  • Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/&u;g
  • Sensitive to CpG methylation
  • SgfI is inactivated by freezing at -70°C
  • Ends generated by SgfI can be directly ligated to ends generated by PvuI. The PvuI site is regenerated in the ligation product, but the SgfI site is not
Specifications

Specifications

SgfI
8 to 12U/μL
1 x 1mL 10X Buffer C, 1 x 150μL Bovine Serum Albumin, Acetylated, 1 x 250U SgfI
7.4
Provides a higher level of quality control for Enzymes used in cloning applications
−30 to −10°C
Liquid
GCGAT.CGC CGC.TAGCG
37°C
250U
10mM Tris HCl (pH 7.4), 300mM KCl, 0.1% Thesit™, 0.1mM EDTA, 1mM DTT, 0.5mg/mL BSA, 50% Glycerol
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