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Promega PureYield™ Plasmid Midiprep System 
PureYield Plasmid Midiprep System provides a fast method for isolating transfection-quality plasmid DNA. Yields 100-200ug of plasmid DNA from 50ml bacteria culture.
Supplier: Promega A2495
Includes: Midiprep Systems (Mfr. Nos. A2492, A2495, A2496) include cell lysis solution, cell resuspension solution, column wash, endotoxin removal wash, neutralization solution, nuclease-free water, PureYield binding columns, PureYield clearing columns. Start-Up Kit (Mfr. No. A6740) also includes Welch™ Vacuum Pump, 110V; Vac-Man™ Laboratory Vacuum Manifold, 20-sample capacity; and four Eluator™ Vacuum Elution Devices.
Description
- Up to 200μg of Transfection-Ready Plasmid DNA from 50mL Cultures
- 30-minute, vacuum-based protocol
- Highly pure, concentrated plasmid DNA
- No tedious, gravity-drip columns or post-elution alcohol precipitation
Specifications
| 15°C to 30°C | |
| Isolates high-quality plasmid DNA from bacterial cultures | |
| 100 Preps | |
| 50mL |
| Spin or Vacuum | |
| 315mL Cell resuspension solution, 315mL Cell lysis solution, 500mL Neutralization solution, 315mL Endotoxin removal wash, 2 × 381mL Column wash, 4 × 25mL Nuclease-free water, 100 PureYield binding columns, 100 PureYield clearing columns | |
| Plasmid Midiprep System | |
| 100 to 200μg |
U.S. Patent 6,194,562, Australian Patent 740145, Canadian Patent 2,329,067.