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Promega™ Proteasome-GLO™ Cell-Based Assays

Homogeneous, luminescent assays that individually measure the chymotrypsin-like, trypsin-like and caspase-like protease activities associated with the proteasome complex in cultured cells

$656.98 - $2,057.61

Specifications

Buffer Proteasome-Glo™ cell-based buffer
For Use With (Application) Measures Chymotrypsin-like Protease activity in cultured cells
Includes Proteasome-Glo™ cell-based Buffer, Z-nLPnLD-Glo™ Substrate and Luciferin detection reagent
Storage Requirements -20°C
View More Specs
Includes:  Buffer, substrate and luciferin detection reagent
Products
Catalog Number Mfr. No. Quantity Price Quantity    

PR-G8861

 
promega™
G8861
500 Assays at 100μL/assay in 96-Well plates or 2000 assays at 25μL/assay in 384-Well plates Each for $2,057.61

PR-G8761

 
promega™
G8761
50mL Each for $2,057.61

PR-G8860

 
promega™
G8860
100 Assays at 100μL/assay in 96-Well plates or 400 assays at 25μL/assay in 384-Well plates Each for $656.98
Description & Specifications

Specifications

Buffer Proteasome-Glo™ cell-based buffer
For Use With (Application) Measures Chymotrypsin-like Protease activity in cultured cells
Includes Proteasome-Glo™ cell-based Buffer, Z-nLPnLD-Glo™ Substrate and Luciferin detection reagent
Storage Requirements -20°C

The luminogenic substrates used to monitor specific protease activities include Suc-LLVY-Glo™ aminoluciferin for chymotrypsin-like, Z-LRR-Glo™ aminoluciferin aminoluciferin for trypsin-like, and Z-nLPnLD-Glo™ aminoluciferin for caspase-like activity. Each luminogenic substrate is added to a buffer system optimized for its specific proteasome activity and luciferase activity. The reagents are added to cells in culture, the proteasome enzyme cleaves the substrates, releasing luciferin, which is consumed by luciferase, producing “glow-type” luminescence correlating to enzyme activity or inhibition.

  • More Biologically Relevant Results: Obtain activity data directly from a cellular environment
  • Simplified Method: “Add-mix-measure” protocol minimizes handling steps and makes the assays amenable to automation
  • Faster Results: Maximum sensitivity is reached 10 to 30 minutes after reagent addition
  • Greater Sensitivity: Avoids inherent fluorescent background signals, providing excellent signal-to-background readings
  • Miniaturizable: Can be used in 384-well format

Assay system also available separately (Cat. No. PRG1200).