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Promega pGEM™-T and pGEM™-T Easy Vector Systems DFS Item

Convenient systems for the routine subcloning of PCR products

$247.71 - $499.37

Spécifications

Promoter SP6, T7
Quantity 20 Reactions
For Use With (Application) For the routine subcloning of PCR products
voir les spécifications

Comprend: Vector, Control insert DNA, T4 DNA ligase, Ligation buffer, High efficiency competent cells (Mfr. Nos. A3610 and A1380 only)

Produits 4
Numéro de catalogue Numéro du manufacturier. Vector Restriction Site Prix Quantité  
Numéro de catalogue Numéro du manufacturier. Vector Restriction Site Prix Quantité  
PR-A1360 DFS Item
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Promega
A1360 		pGEM-T easy vector I BstZI, NotI and EcoRI
chaque for $299.09
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PR-A1380 DFS Item
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Promega
A1380 		pGEM-T easy vector II BstZI, NotI and EcoRI
chaque for $499.37
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PR-A3610 DFS Item
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Promega
A3610 		pGEM-T vector II BstZI
chaque for $449.31
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PR-A3600 DFS Item
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Promega
A3600 		pGEM-T vector I BstZI
chaque for $247.71
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Description

Description

The pGEM-T Vector is prepared by cutting Promega's pGEM-5Zf(+) Vector with EcoR V and adding a 3« terminal thymidine to both ends. These single 3«-T overhangs at the insertion site greatly improve the efficiency of ligation of a PCR product into the plasmid.

  • 2X Rapid Ligation Buffer allows reactions to be completed in one hour at room temperature Blue/white screening can be used to directly identify recombinant clones, as T7 and SP6 RNA polymerase promoters flank a multiple cloning region within the α-peptide coding region for β-galactosidase, permitting insertional inactivation of the α-peptide
  • f1 Origin of Replication allows the preparation of single-stranded DNA

pGEM-T Vector Systems

  • Multiple cloning site is flanked by recognition sites for the restriction enzyme BstZ I Single-enzyme digestion allows release of the insert
    • Double digestion may also be used to release the insert from the vector
pGEM-T Easy Vector Systems
  • Recognition sites for BstZ I, EcoR I, and Not I flank the insertion site Several options are available for removal of the desired insert DNA with a single restriction digestion

Spécifications

Spécifications

SP6, T7
20 Reactions
For the routine subcloning of PCR products
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