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Promega ImProm-II™ Reverse Transcription DFS Item

Formulated for stand-alone applications or for easy transition to gene-specific target amplification.

Supplier:  Promega A3800

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Catalog No. PR-A3800


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Description

Description

  • Reverse transcribes RNA templates from total RNA, poly(A)+ mRNA, or synthetic transcript RNA
  • Enables robust, full-length cDNA synthesis for the analysis of rare or long messages (up to 8.9kb)
  • Uses volumes of 1–20μL from the initial RT reaction in coupled or uncoupled PCR reactions
  • May also be used for the incorporation of regular, Cy™3-modified, Cy5-modified, and amino-allyl-modified nucleotides for microarray applications
  • Refill components available separately

Specifications

Specifications

-30°C to -10°C
Upstream control primer 100μL, Downstream control primer 100μL, MgCl2 1.2mL, Oligo(dT)15 Primer 50μg, dNTP mix 320μL, Random primers 50μg, 1.2kb kanamycin positive control RNA 5μg, ImProm-II 5X reaction buffer 600μL, ImProm-II reverse transcriptase 100μL, Recombinant RNasin ribonuclease inhibitor 2500U, Nuclease-free water 2 × 1250μL
7
Liquid
Reverse transcription of RNA templates from total RNA, poly(A)+ mRNA, or synthetic transcript RNA
100
ImProm-II Reverse Transcription System
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