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Thermo Scientific™ POROS™ HQ 50 μm Strong Anion Exchange Resin
Description
POROS™ 50-micron resins are available for low pressure applications, including large scale downstream bioprocessing. POROS HQ is based on a quaternized polyethyleneimine functional group yielding a high capacity, Perfusion Chromatography™ resin designed for the separation and purification of biomolecules.
- Higher productivity—high throughput and high dynamic capacity associated with Perfusion Chromatography
- Chemical stability—allows aggressive cleaning and sanitization
- Enhanced biomolecule access—provided via large pores, ranging between 500-10000 Å
- Polystyrenedivinylbenzene particles—yield a robust, easily packable matrix
- Develop better separations methods in a shorter time frame—the speed of Perfusion Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation
Reduce time-consuming sample prep
You can replace many sample preparation steps with high-speed Perfusion Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.
Create novel assays for more efficient analysis
Perfusion Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.
High capacity, high resolution, high speed
In contrast to conventional chromatography media, POROS Perfusion Chromatography resin particles are engineered to have two discreet classes of pores. Large throughpores allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short diffusive pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased—without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm/hr compared to 50 to 360 cm/hr for conventional resins
Specifications
Specifications
| Type | HQ Strong Anion Exchange Resin |
| Standard Components | Quaternized Polyethyleneimine |
| For Use With (Application) | Separation and Purification of Biomolecules |
| Quantity | 10 mL |
| Volume (Metric) | 10 mL |
| Content And Storage | Store in refrigerator (2–8°C). |
| Product Line | POROS |
| Format | Poros Bead |
| Stationary Phase | Quaternized Polyethyleneimine |
| Purification Target | Protein |
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Frequently Asked Questions (FAQs)
All of the pre-packed POROS columns, regardless of column size or resin, have the same 10-32 female fittings on the column. Columns are compatible with Waters and Agilent HPLC systems. You will need 1/16 inch male fittings for the HPLC to attach the column.
All of the pre-packed columns, regardless column size or resin, have the same 10-32 female fittings on the column. They are all compatible with the AKTA instrument platform, however due to the column volume of the 2.1 mmD x 30 mmL columns (0.1 mL), these will not perform well in AKTA or Avant purification systems due to the relative size of the hold- up volume in these systems. The smallest column (PEEK or SS) that could feasibly be used in these systems is the 4.6mmD x 50mmL (0.8 mL) format.
The physical stability of POROS resins is high. Customers have agitated, with overhead mixing and or bottom gassing for long periods of time (5-10 hours) and have performed hundreds of diaphragm pump passes during stall packs. Keep in mind that agitation with any device that creates grinding and sheer, such as with a magnetic stir bar, is not recommended for any type of resin.
POROS resins rehydrate quite well. Here is the procedure that we recommend:
1. Flow 5% ethanol over the column at ?350 cm/hour for 3 CVs, followed by water or 0.1 M sodium chloride at 300 cm/hour for 3 CVs.
2. If baseline noise is still present, flow 20% ethanol over the column at 300 cm/hour for 3 CVs, followed by water or 0.1 M sodium chloride at 300 cm/hour for 3 CVs.
3. If you have established column qualification tests and specifications, perform the tests after rewetting.
In general POROS resins have behaved better for large biomolecules such as viral particles, fusion proteins, and globular proteins, where shape (not size) matters due to its unique pore structure and large pore structure as compared to traditional resins. As the target molecule size increases, capacities obtained will decrease. The large pore structure of POROS resins, which allows for convective flow (and therefore enhanced diffusion) is especially well suited for the purification of large biomolecules. POROS resins offer the best blend of high capacity (associated with chromatography beads) and improved chromatography efficiency (typically associated with monoliths or membranes). The average pore size for POROS resins is 1,000-3,600 Angstrom (100-360 nm) depending on the base bead/chemistry being utilized. For example, the molecular weight of IgG is 150 kDa and the molecular radius is 55 Angstrom. The molecular weight of IgM is ~900 kDa (pentamer), and the molecular radius is 120 Angstrom. So both of these biomolecules can interact with the pore structure associated with POROS materials.
For Research Use Only. Not for use in diagnostic procedures.