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Molecular Probes™ Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit

Catalog No. A24881
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40 tests
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A24881 40 tests
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Catalog No. A24881 Supplier Molecular Probes™ Supplier No. A24881
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Description

Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit

The Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit enables optimal image-based analysis of four key markers of human pluripotent stem cells (PSCs): OCT4, SOX2, SSEA4, and TRA-1-60. This high performance immunocytochemistry (ICC) kit includes a complete set of primary and secondary antibodies, a nuclear DNA stain, and premade buffers for an optimized staining experiment. To characterize pluripotent stem cells using only two markers, we recommend us of the Pluripotent Stem Cell Immunocytochemistry Kit (OCT4, SSEA4) or the Pluripotent Stem Cell Immunocytochemistry Kit (SOX2, TRA-1-60).

With the Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit, you can:
  • Confirm expression of key pluripotent stem cell markers with highly specific antibodies
  • Get more information per sample by measuring more than one marker at a time
  • Generate beautiful multiplexed images with minimal effort
  • Save time and avoid cell loss by minimizing unnecessary wash steps in your staining protocol
  • Process samples with confidence using a complete and optimized set of ICC reagents

    Confirm Expression of Key PSC Markers
    The antibodies included in the kit have been carefully selected and validated for high performance in the ICC analysis of PSCs, whether cultured under feeder-dependent or feeder-free media systems. OCT4 and SOX2 are critical nuclear markers, while SSEA4 and TRA-1-60 are important cell surface markers.

    Get More Information Per Sample
    The antibody combinations in the kit have been designed to allow for the specific and simultaneous assessment of two markers at a time (e.g., OCT4 & SSEA4 and SOX2 & TRA-1-60) along with nuclear DNA staining to help save time and precious sample.

    Generate Beautiful Multiplexed Images
    Powered by the superior performance of bright, photostable Alexa Fluor™ dyes, this kit enables the acquisition of beautiful multiplexed images of PSC marker expression using commonly available blue, green, and orange/red filter sets.

    Minimize Unnecessary Wash Steps
    The streamlined method recommended for this kit eliminates unnecessary wash steps found in standard ICC protocols to help save time and decrease the likelihood of cell loss during the staining protocol, especially when using glass coverslips or chamber slides. The only washes required are after incubation with primary and secondary antibodies.

    Process Samples with Confidence
    An optimized set of premade fixation, permeabilization, blocking, and wash buffers allows you to focus on answering scientific questions rather than spending time preparing reagents and determining compatibility.

    What You Get
    Easily scaled to any culture plate, dish, or slide format, the Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit comes with reagents sufficient to stain 40 samples using a 50 uL staining volume. (See kit content details below.)

    • Specifications

    Conjugate 2 Color, Alexa Fluor 488, Alexa Fluor 555, Alexa Fluor 594
    Quantity 40 tests
    Content And Storage Store at -20°C to 4°C:
  • 10 μL of Rabbit Anti-Human OCT4 (200X)
  • 20 μL of Rat Anti-Human SOX2 (100X)
  • 20 μL of Mouse Anti-Human SSEA4 (100X)
  • 20 μL of Mouse Anti-Human TRA-1-60 (100X)
  • 20 μL of Alexa Fluor™ 555 Donkey Anti-Rabbit (250X)
  • 20 μL of Alexa Fluor™ 594 Donkey Anti-Rabbit (250X)
  • 20 μL of Alexa Fluor™ 488 Donkey Anti-Rat (250X)
  • 20 μL of Alexa Fluor™ 555 Goat Anti-Mouse IgM (250X)
  • 20 μL of Alexa Fluor™ 594 Goat Anti-Mouse IgM (250X)
  • 20 μL of Alexa Fluor™ 488 Goat Anti-Mouse IgG3 (250X)

    Store at -20°C to RT:
  • 1 vial of NucBlue™ Fixed Cell ReadyProbes™ Reagent (1X)
  • 10 mL of Fixative Solution (1X)
  • 10 mL of Permeabilization Solution S (1X)
  • 20 mL of Blocking Solution (1X)
  • 20 mL of Wash Buffer (10X)
    		•
    Product Type Immunocytochemistry Kit

    Frequently Asked Questions (FAQs)

    The Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit provides the NucBlue Fixed Cell Stain. If I don't have the correct filter set for this stain, can I use another nucleic acid stain?

    If you use Alexa Fluor 488/Alexa Fluor 555 dye-conjugated secondary antibodies and you have a Cy5/Alexa Fluor 647 filter set, you may use a far-red nucleic acid stain, such as NucRed Dead 647 ReadyProbes Reagent (Cat. No. R37113) or SYTOX Deep Red Nucleic Acid Stain (Cat. No. S11380). Do not use these far-red nucleic acid stains if using an Alexa Fluor 594 dye-conjugated secondary antibody as there is too much spectral overlap of Alexa Fluor 594 into the Cy5/Alexa Fluor 647 range.

    Can I use the Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit for flow cytometry analysis?

    We do not recommend using the Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit for flow cytometry analysis. The kit and protocol were specifically developed for imaging analysis (microscopy/high-content screening (HCS)).
    Do you offer the individual components of the Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit as standalone products?

    Only the NucBlue Fixed Cell ReadyProbes Reagent may be purchased separately as Cat. No. R37606. We do offer the secondary antibodies separately, but not in the same proprietary concentration as provided in the kit. We also offer formaldehyde, DPBS, BSA, and saponin as separate catalog numbers, but not in the volumes or as formulated in the kit. The primary antibodies are not available as separate stock items; all non-stock items in the kit may be available as a custom order. Please contact your Account Representative.

    For Research Use Only. Not for use in diagnostic procedures.