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Thermo Scientific™ Yeast beta-Galactosidase Assay Kit

Catalog No. PI75768
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75 mL
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PI75768 75 mL
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Catalog No. PI75768 Supplier Thermo Scientific™ Supplier No. 75768

Description

Extract protein from yeast two-hybrid reporter systems and measure beta-galactosidase activity using compatible Y-PER Reagent and b-Gal Assay Reagent.

Thermo Scientific™ Yeast β-Galactosidase Assay Kit is specially formulated for yeast cells and ideal for identifying protein interactions in vivo using two-hybrid systems.

The Yeast β-Galactosidase Assay Kit allows for qualitative or quantitative determination of β-galactosidase activity in solution directly from colonies growing on solid medium. A portion of the colony is suspended in a mixture of Thermo Scientific Y-PER™ Yeast Protein Extraction Reagent and the β-Galactosidase Assay Buffer. After a brief incubation period, the solution turns yellow from the hydrolysis of o-nitrophenyl-β-D-galactopyranoside (ONPG) to o-nitrophenol (ONP) and galactose in a mildly alkaline solution. The assay becomes quantitative if calibrated to cell number by measuring the optical density at 660nm.

Highlights:

  • Efficient lysis of yeast cells and a colorimetric detection system
  • Quantitative or qualitative assay for yeast colonies grown on solid media or in suspension
  • Colorimetric assay easily measured in a plate reader or spectrophotometer (420nm)
  • Adaptable to 96-well microplates or tube assay formats
  • Perform homogeneous screening assays or measure activity secondarily after cell lysis
  • Also compatible with prepared lysates from other species such as bacteria

Includes:

Thermo Scientific Y-PER Yeast Protein Extraction Reagent (Part No. 78990), 2X Assay Buffer and Stop Solution

Specifications

Content And Storage Sufficient For: 250 colonies or 250 culture samples (100 μL each)
  • Y-PER Yeast Protein Extraction Reagent, 25 mL (store at room temperature)
  • 2X beta-Galactosidase Assay Buffer, 25 mL (store at -20°C)
  • 1M Sodium Carbonate Stop Solution, 25 mL (store at room temperature)
    		•
    Detection Method Absorbance
    For Use With (Application) Absorbance
    For Use With (Equipment) Microplate Reader
    Product Type Beta-Galactosidase Assay Kit
    Dye Type Enzyme Labeled
    Format 96-well Plate
    Product Line Pierce
    Quantity 75 mL
    Substrate ONPG
    Substrate Type Beta-Gal Substrate
    Sufficient For 250 colonies or 250 culture samples (100 μL each)
    Target Beta-Galactosidase
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    For Research Use Only. Not for use in diagnostic procedures.