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Thermo Scientific™ Sulfo-EGS (ethylene glycol bis(sulfosuccinimidyl succinate))
Crosslink amines to amines with a crosslinker composed of sulfo-NHS-ester groups and hydroxylamine-cleavable spacer arm; water-soluble for cell-surface protein crosslinking.
Supplier: Thermo Scientific™ LSG21566
Description
Thermo Scientific™ Pierce Sulfo-EGS is a water-soluble crosslinker that contains amine-reactive sulfo-NHS-ester ends around a 12-atom spacer arm, which can be cleaved by treatment with hydroxylamine at pH 8.5.
Highlights:
- Water-soluble
- Cleavable at pH 8.5 using hydroxylamine and heat
- Lactose dehydrogenase retained 60% of its activity after reversible crosslinking
- Reactive groups: sulfo-NHS esters (homobifunctional)
- Reactive toward: amino groups
Specifications
| Amine-Amine | |
| Sulfo-EGS | |
| No | |
| Upon receipt store desiccated at -20°C. | |
| Ambient | |
| Chemical Labeling | |
| Sulfo-NHS Ester | |
| Powder | |
| Water | |
| Yes |
| By Hydroxylamine | |
| 660.45 | |
| 16.1 AÌ | |
| No | |
| Pierce™ | |
| Homobifunctional | |
| Medium (10 to 30 AÌ) | |
| 50 mg | |
| Standard | |
| Crosslinker |
For Research Use Only. Not for use in diagnostic procedures.