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Thermo Scientific™ Powdered RPMI Media for SILAC

Catalog No. PI88426
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PI88426 104 g
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Catalog No. PI88426 Supplier Thermo Scientific™ Supplier No. 88426
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Description

Analyze protein expression by mass spectrometry using these media or additives for stable isotope labeling with amino acids in cell culture (SILAC).

These Thermo Scientific Pierce cell culture media and serum components are optimized for use with stable isotope labeling with amino acids in cell culture (SILAC) to analyze protein expression by mass spectrometry (MS).

SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of heavy 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.

Highlights:

  • Flexible–liquid media deficient in both L-lysine and L-arginine and powdered media deficient in L-leucine, L-lysine and L-arginine, allowing for more complete proteome coverage through multiple isotopic amino acid labeling

  • Compatible–DMEM, MEM, RPMI-1640, IMDM, Ham's F12 and McCoy's 5A media and dialyzed FBS are used to culture a variety of cell lines, including HeLa, 293T, COS7, U2OS, A549, A431, HepG2, NIH 3T3, K562 and Jurkat

  • Optimized, specialized media–the Expansion DMEM and Low-Osmolarity DMEM are specifically designed and qualified for mouse embryonic stem cell propagation and SILAC protein expression profiling with mass spectrometry

  • High quality–Stem Cell Screened Dialyzed FBS is tested to ensure that it does not promote stem cell differentiation

Specifications

Formulation RPMI media minus L-Leucine, L-Lysine and L-Arginine
For Use With (Equipment) Mass Spectrometer
Product Type Media
Content And Storage Store in refrigerator (2 to 8°C).
Shipping Condition Wet Ice
Starting Material Cells
Final Product Type Protein
Workflow Step Protein Labeling
Quantity 104 g
Form Powder

Frequently Asked Questions (FAQs)

Do you offer any heavy amino acids other than the ones in the SILAC Protein Quantitation Kits?

We offer additional isotopes of lysine, including 4,4,5,5 D4 L-Lysine-2HCl (Cat. No. 88437) and 13C6 15N2 L-Lysine-2HCl (Cat. No. 88209), which can be used for multiplexed experiments. 13C6 L-Arginine-HCl (Cat. No. 88210) and 13C6 15N2 L-Arginine-HCl (Cat. No. 89990) are also available to enhance peptide isotope label coverage of tryptic peptides. In addition to lysine and arginine, 13C6 L-Leucine (Cat. No. 88435) can also be used with our powdered SILAC DMEM (Cat. No. 88425) and RPMI-1640 media (Cat. No. 88426). Please check the product page for a current listing.

For Research Use Only. Not for use in diagnostic procedures.