Thermo Scientific™ Pierce™ Recombinant Protein G and Conjugates
Detect mouse and human antibodies, especially IgG isotypes, in immunoassays with this purified, biotinylated or peroxidase-conjugated recombinant Protein G.
Manufacturer: Thermo Scientific™ PI31499
Purified and conjugated forms of Thermo Scientific™ Pierce Recombinant Protein G enable probing and detection of mouse and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.Protein G is a bacterial cell wall protein isolated from group G Streptococci. DNA sequencing of native Protein G identifies two IgG-binding domains and sites for albumin and cell surface binding. The albumin and cell surface binding domains have been eliminated from Recombinant Protein G to reduce nonspecific binding and, therefore, can be used to separate IgG from crude samples. Optimal binding occurs at pH 5, although binding is also effective at pH 7.0 to 7.2.
- Contains two Fc-binding domains per protein
- Better than Protein A for mouse (incl. IgG1), human (incl. IgG3) and rat, goat and cow antibodies
- Poorer than Protein A for guinea pig, pig, dog and cat
- Does not bind human IgM, IgD or IgA
|Lyophilized powder from protein in buffered solution|
|Pierce Recombinant Protein G, Peroxidase Conjugated|