missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Antibody Production and Purification
  5. Antibody Isotyping and Characterization
  6. PI26179

Thermo Scientific™ Pierce™ Rapid Antibody Isotyping Kit plus Kappa and Lambda - Mouse

Catalog No. PI26179
Change view
Click to view available options
Quantity:
10 Tests
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
PI26179 10 Tests
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. PI26179 Supplier Thermo Scientific™ Supplier No. 26179
Only null left

Description

Identify mouse monoclonal antibody class and subclass in 5 minutes with these single-use, cassette-based lateral-flow assays.

Thermo Scientific™ Pierce Rapid Mouse Antibody Isotyping Kits are single-use, cassette-based kits for 5-minute lateral-flow assay determination of mouse monoclonal antibody class and subclass identity.

The kits are composed of small cartridges that provide a color-readout of the monoclonal antibody isotype within five minutes after pipetting a small amount of diluted culture supernatant or ascites fluid sample into the sample well. The kits determine mouse IgG1, IgG2a, IgG2b, IgG3, IgA, IgM.

The assay is performed by simply adding a properly diluted tissue culture supernatant or mouse ascites sample to the well of the small cassette. Gold conjugates embedded in the cassette form specific class- and subclass-soluble complexes with the antibodies in the sample. These complexes travel the length of the membrane and are resolved on the anti-isotype and class-specific antibody-impregnated membrane. Results are displayed as a red band indicating the antibody isotype or subclass.

Highlights:

  • Long shelf life – stable for more than 1 year at room temperature

  • Single-step – add diluted antibody sample to the loading-well of cassette

  • Fast – within 5 minutes a band appears that indicates the antibody isotype

  • Sensitive – detect antibodies in tissue culture media or ascites fluid samples at any concentration greater than 10ng/mL

  • Specific – assay does not crossreact with fetal bovine serum (FBS)

  • Reliable – results are similar to the standard ELISA-based isotyping assays

Includes:

Ten pouches, each containing two (Part No. 26178) or three (Part No. 26179) cassettes, plus a bottle of sample diluent

Specifications

Description Pierce Rapid Isotyping Kit plus Kappa and Lambda - Mouse
Product Line Pierce
Target Specificity Target-specific
Quantity 10 Tests
For Use With (Application) Membrane Blot
Assay Isotyping Assay
Detection Method Chromogenic
Content And Storage Sufficient For: 10 isotype determinations
• Isotyping Cassettes (three per pouch), 10 pouches
• Sample Diluent, 45 mL

Store kit at 2–8°C.

Frequently Asked Questions (FAQs)

How does the Rapid ELISA Mouse Antibody Isotyping kit (Cat. No. 37503) differ from the Cassette-based Kits (Cat. Nos. 26178 and 26179)?

The ELISA Kit procedure takes approximately 1.5 hours to complete and enables simultaneous testing of multiple samples (12 per plate). The Cassette-based Kits provide results in only 5 minutes but are single-use units that are less practical and more costly per sample than the ELISA Kit. The Cassette-based Kits are less sensitive (minimum antibody concentration is 10 ng/mL) and provide only qualitative assessment.
Can I use the Pro-Detect assay kits for rapidly isotyping antibodies?

The Pro-Detect Rapid Antibody Isotyping Kit is a lateral-flow assay with high sensitivity for rapid determination of the immunoglobulin class, subclass, and light chain type of monoclonal antibodies. Two different formats of rapid isotyping kits are available. These include dipstick format and cassette-based format. One can use these kits to identify the isotype of a mouse, rat, or human antibody. Make sure to follow the recommended dilution guidelines.

I am using a Pro-Detect assay kit and cannot differentiate banding pattern between control lysates and lysates that are actually expressing proteins of interest. What is the problem?

1) It is likely that there could be a substance interfering with the assay. Check the compatibility table on this page (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/pro-detect-rapid-assays.html), under “Detergent and salt compatibility limits”.
2) The expression level of the actual tagged protein is below the detectable range of the assay.

Using Pro-Detect assay kits, can I detect the protein of interest in a mixture of proteins such as a crude lysate?

Yes, first, make sure that the protein of interest is tagged and that we offer a Pro-Detect assay kit that can detect that tag. Secondly, make sure that you follow the guidelines to detect proteins in crude lysate and if necessary increase or decrease the lysate concentration to bring the protein of interest within the detectable range of the assay.

Note that the guidelines for the recommended detection ranges reflect the concentration of the pure tagged protein in a given sample mixture.
How do I interpret the gradual disappearance of four lines in the Pro-Detect competition assay kits?

As the concentration of the tag in the protein sample increases, the number of lines will decrease until they all disappear. The concentration of the tagged proteins is inversely related to the number of lines appearing on the strip.
Are the results of Pro-Detect assay kits comparable to western blotting?

Yes. Western blotting is a widely used and accepted tool to identify a specific protein in a complex mixture. However, it is a time-consuming and labor-intensive process, with many preparative steps and time intervals to manage. The Pro-Detect rapid assays are able to provide comparable accuracy but with much shorter processing time (10-15 min) and less hands-on steps. For further information, please see the data shown on this page (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/pro-detect-rapid-assays.html) under “Results are comparable to western blotting”.
How specific are the Pro-Detect assay kits? Is there any cross-reactivity observed among various kits ?

Except for MBP Pro-Detect strips which cross-react with bacterial proteins, no other cross-reactivity of Pro-Detect strips has been observed. An example is shown in the figure on this page (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/pro-detect-rapid-assays.html) under “No cross-reactivity with other targets” where there was no cross-reactivity of Pro-Detect rapid GST assay strips with proteins endogenous to mammalian, bacterial, Sf9 cells (lanes 1-5) or with any other non-specific tag proteins (lanes 7-19). In addition, even when using 100% of Minimum Essential Medium with 10% FBS, there was no cross-reactivity detected.
Are the Pro-Detect assay kits compatible with common detergent and salt concentrations used in protein purification/detection protocols?

We examined the performance of the Pro-Detect rapid assay in the presence of various concentrations of detergents and salts that are commonly used in protein expression and isolation. Reagents tested include NaCl, Urea, Triton-X 100, SDS, NP40, EDTA, Glycerol, KCl, CHAPS, RIPA, BPER, and MPER. For estimates of each assay tolerance level for the tested detergents and salts, please refer to the table under “Detergent and salt compatibility limits” at https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/pro-detect-rapid-assays.html

What is the ideal range of protein concentration needed for using Pro-Detect assay kits?

For sandwich assays, the range is 0.1-10 µg/mL of pure tagged protein.
Note: The lateral flow assays can detect outside of this range; however, intensity of the bands will be lower at concentrations higher and lower than the working range. It is not recommended to use at concentrations less than 0.01 µg/mL. In a mixture of proteins such as lysates, the target protein has to be in the detectable range (0.1-10 µg/mL) for the assay to be successful.

For competition assays, the range is 4-20 µg/ml.
Note: The competitive lateral flow assays can detect down to 1 µg/mL, but loss of band intensity may be less distinguished. As there is no upper limit, the lateral flow strip will continue to show positive results at concentrations greater than 20 µg/mL, visualized by complete loss of test lines (test lines closest to the sample will disappear first). It is not recommended to use at concentrations less than 1 µg/mL.

For Research Use Only. Not for use in diagnostic procedures.