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  6. Thermo Scientific™ Pierce™ Protein A/G Magnetic Beads

Thermo Scientific™ Pierce™ Protein A/G Magnetic Beads

Catalog No. PI88803
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PI88803 5 mL
PI88802 1 mL
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Catalog No. PI88803 Supplier Thermo Scientific™ Supplier No. LSG88803
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Description

Immunoprecipitate or affinity purify antibodies (IgG) using Protein A/G coated magnetic particles for manual or robotic magnetic separation.

Thermo Scientific Pierce Protein A/G Magnetic Beads are high-performance affinity particles for antibody purification and immunoprecipitation methods using manual or robotic magnetic separators.

Features of Protein A/G Magnetic Beads:

  • High capacity—nearly four times higher binding capacity than typical magnetic beads from other suppliers, allowing the use of smaller amounts per experiment
  • Low non-specific binding—stable, pre-blocked beads provide clean purification products (e.g., antigen eluted in IP with antibody is devoid of contaminating proteins from complex IP matrix)
  • Flexibility—convenience of IgG binding domains of both Protein A and Protein G on one bead
  • Compatibility—beads are compatible with manual and automated applications (e.g., Thermo Scientific KingFisher Instruments)
  • Assay consistency—magnetic beads eliminate resin loss and provide for more efficient separation of solutions than traditional IP methods that use only microcentrifuge tubes

These magnetic beads are coated with genetically engineered Pierce Protein A/G, a recombinant fusion protein which combines the IgG binding domains of both Protein A and Protein G. This enables capture of antibodies from a wider range of species and isotypes than either protein alone. Using our crosslinker chemistry, you can immobilize an antibody onto the magnetic particle and prevent IgG contamination in your immunoprecipitated sample. These beads can be used both manually with a magnetic stand as well as with automated platforms such as the Thermo Scientific KingFisher Instruments.

Applications:

  • IP and Co-IP experiments (see complete kit)
  • Immunoprecipitation for analysis in non-reducing conditions
  • Antibody purification

The recombinant Protein A/G that is immobilized onto the Pierce Magnetic Beads is a fusion of the IgG binding domains of both Protein A and Protein G. Protein A/G contains four Fc-binding domains from Protein A and two from Protein G, making it a convenient tool for investigating and purifying immunoglobulins. Thus, Pierce Magnetic Particles are not simply a mixed immobilization of separate Protein A and Protein G polypeptides, nor are they a mixture of Protein A magnetic beads and Protein G magnetic beads.

Specifications

Capacity (Metric) 5 mL
Concentration Slurry: 10 mg/mL, 1% solids
Content And Storage Upon receipt store at 4°C. Product shipped with an ice pack.
Particle Size 1 μm
Form Liquid
Product Line Pierce
Quantity 5 mL
Protein Form Recombinant
Target Antibody
Type Magnetic Affinity Bead
Ligand Type Protein A/G
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Frequently Asked Questions (FAQs)

Can I reuse Pierce Protein A/G Magnetic Beads (Cat. No. 88802, 88803)?

Pierce Protein A/G Magnetic Beads are intended for single use only. Therefore, we do not recommend reusing them.
What advantage do Pierce Magnetic Agarose beads have over Dynabeads or Pierce Magnetic Beads?

Pierce Magentic Agarose Beads have a much higher binding capacity.
Can I centrifuge the Thermo Scientific Protein A/G Magnetic Beads?

No, centrifuging causes the formation of irreversible aggregates which greatly reduces binding capacity.
How do I reduce non-specific binding to Thermo Scientific Protein A/G Magnetic Beads?

Adding 50-350 mM of NaCl to the Binding/Wash and Elution Buffers can help reduce non-specific bands. Also, use a low-pH elution for single-use applications. The optimal time for low-pH elution is 10 minutes; exceeding 10 minutes may result in non-specific binding and yield reduction.
When should I avoid boiling the Thermo Scientific Protein A/G Magnetic Beads?

Avoid bead-boil elutions when using rabbit antibodies (primary or secondary) in downstream Western blot applications. Instead, perform elution in SDS-PAGE sample buffer at room temperature. For all other antibody species, boiling the beads in SDS-PAGE sample buffer is acceptable for single-use applications. Boiling could cause bead aggregation and loss of binding activity.
How can I prevent bead aggregation with Thermo Scientific Protein A/G Magnetic Beads?

Centrifuging, drying or freezing will cause the beads to aggregate and lose binding activity. To ensure good dispersal of beads for optimal antibody binding, it is important to include 0.05% non-ionic (e.g., Tween-20 Detergent) or zwitterionic (e.g., CHAPS) detergent in the binding buffer and mix the beads during incubation.
Will the Thermo Scientific Protein A/G Magnetic Beads bind to goat IgG?

Yes, Protein A/G magnetic beads will bind to goat IgG as well as several other species of IgG such as rabbit, mouse, etc. For details of species as well as different immunoglobulin isotypes that will bind to Protein A/G, please look at this Tech Tip (http://tools.thermofisher.com/content/sfs/brochures/TR0034-Ab-binding-proteins.pdf).
What are the differences between Protein A, Protein G, Protein A/G, and Protein L Ig-binding proteins?

Protein A, Protein G, and Protein A/G bind almost exclusively to the IgG class of antibodies, but their binding properties differ among species and subclasses of IgG. Protein L binds in the variable fragment of some kappa light chains and can react with any immunoglobulin, not just IgG, as long as the correct kappa light chains are present. Protein L does not bind lambda light chains and certain kappa chains of different species.

-Protein A is generally preferred for rabbit, pig, dog, and cat IgG.
-Protein G has better binding capacity for a broader range of mouse and human IgG subclasses (e.g., IgG1 vs. IgG2)
-Protein A/G is a recombinant fusion protein that includes the IgG-binding domains of both Protein A and Protein G. Therefore, Protein A/G is ideal for binding the broadest range of IgG subclasses from rabbit, mouse, human, and other mammalian samples.
-Protein L binds to certain immunoglobulin kappa light chains. Because kappa light chains occur in members of all classes of immunoglobulin (i.e., IgG, IgM, IgA, IgE and IgD), Protein L can purify these different classes of antibody. However, only those antibodies within each class that possess the appropriate kappa light chains will bind. Generally, empirical testing is required to determine if Protein L is effective for purifying a particular antibody. It binds only Vk1 in mouse and VkI, VkIII and VkIV in human.
Read more about the general characteristics of Ig-binding proteins (https://www.thermofisher.com/us/en/home/life-science/antibodies/antibody-purification-kits-reagents.html) and (https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/TR0034-Ab-binding-proteins.pdf).

For Research Use Only. Not for use in diagnostic procedures.