missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Protein Purification
  5. Protease Inhibitors and Phosphatase Inhibitors
  6. PIA32963

Thermo Scientific™ Pierce Protease Inhibitor Tablets

Catalog No. PIA32963
Change view
Click to view available options
Quantity:
20 Tablets
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
PIA32963 20 Tablets
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. PIA32963 Supplier Thermo Scientific™ Supplier No. A32963
Only null left

Description

Thermo Scientific™ Pierce Protease Inhibitor Tablets and Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments.

Thermo Scientific™ Pierce Protease Inhibitor Tablets and Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments.

Additional Information and Online Ordering

Pierce Protease Inhibitor Tablets protect proteins from proteolytic degradation through inhibition of the serine-proteases, cysteine-proteases, aspartic acid-proteases and aminopeptidases that are typically present in cellular lysate samples. The tablets contain AEBSF, aprotinin, bestatin, E-64, leupeptin and pepstatin A and are available in formulations with or without EDTA, a metalloproteinase inhibitor. The EDTA-free formulation has added compatibility with IMAC purification methods and 2D gel electrophoresis. Stable for storage at 4°C for up to 12 months, each large tablet is sufficient for 50mL of solution and each mini tablet is sufficient for 10mL of solution. Both sizes and formulations of tablet are suitable for reagent preparation prior to protein extraction from tissue and cultured cells.

Highlights:

  • Economical −both large and mini tablets are most cost effective than other commercially available formulations for equivalent volumes
  • Easy−to−use − the refrigerator−stable tablets are easily dissolved in buffers and lysis reagents
  • Two formulation − available with or without EDTA
  • Compatible − use with homebrew or commercially available cell lysis reagents and Thermo Scientific Protein Assays

Specifications

Description Pierce Protease Inhibitor Tablets
Product Type Protease Inhibitor
Content And Storage 1 Vial, Store at 2°C to 8°C
Shipping Condition Room Temperature
Form Solid
Product Line Pierce
Sufficient For 50 mL Solution
Inhibitors Protease Inhibitor
Quantity 20 Tablets

Frequently Asked Questions (FAQs)

Do Pierce Protease Inhibitor Tablets (Cat. No. A32963) dissolve quickly?

Pierce Protease Inhibitor Tablets (Cat. No. A32963) dissolve quickly to form a clear solution. We recommend dissolving 1 tablet in 50 mL of buffer or lysate. The protease inhibitor formulation is compatible with most detergent-based cell lysis reagents, including Pierce Cell Lysis solutions. Pierce Protease Inhibitor Tablets do not contain phosphatase inhibitors. If necessary, phosphatase inhibitors, such as Pierce Phosphatase Inhibitor Mini Tablets (Cat. No. A32957) can be added along with the protease inhibitor tablets.
Can I use just part of the Pierce Protease Inhibitor XL Capsules, EDTA-Free and save part of the capsule for later use?

We do not recommend “splitting” the capsule into smaller portions. Researchers using less than 500 mL should choose a different format (liquid or tablet) of protease inhibitors.
What formats of protease inhibitors do you offer?

We offer standalone protease inhibitors (AEBSF, Aprotinin, PMSF, EDTA, etc.), as well as cocktails or combinations of protease inhibitors in liquid, tablet, and larger capsule formats. These cocktails of protease inhibitors can also be purchased in combination with a cocktail of phosphatase inhibitors, in both liquid and tablet form, for even greater convenience.
Do you offer any of the components of the Pierce protease, phosphatase and (protease and phosphatase) inhibitor tablets and mini tablets individually?

Yes, the following components of the Pierce protease, phosphatase and (protease and phosphatase) inhibitor tablets and mini tablets are offered separately:

- AEBSF (Cat. No. 78431)
- Aprotinin (Cat. No. 78432)
- Bestatin (Cat. No. 8433)
- E-64 (Cat. No. 78434)
- Leupeptin (Cat. No. 78435)
- Pepstatin A (Cat. No. 78436)
- EDTA (Cat. No. 17892).
What are the concentrations of the various components of the protease and phosphatase inhibitor tablets and mini tablets?

The final concentration of each component in the protease and phosphatase inhibitor tablets and mini tablets is proprietary. We release only the identities of the inhibitors.
Can I store the reconstituted solution of protease, phosphatase and (protease and phosphatase) inhibitor tablet or mini tablet and if so, for how long?

The Pierce protease, phosphatase, as well as (protease and phosphatase) inhibitor tablets and mini tablets are stable for at least one year when stored at 4 degrees C, but we only recommend preparing fresh solutions just before needed. If you are interested in liquid protease or phosphatase inhibitors, please use our inhibitor cocktails (https://www.thermofisher.com/search/results?query=protease%20inhibitor%20cocktails&focusarea=Search%20All).
Which protein assay is compatible with the protease, phosphatase, as well as (protease and phosphatase) inhibitor tablets and mini tablets?

The Pierce protease, phosphatase and (protease and phosphatase) inhibitor tablets and mini tablets are compatible with common protein assays such as BCA Protein Assay, 660 nm Protein Assay, Coomassie Plus (Bradford) Assay, and Detergent Compatible Bradford Assay.
When do I need to use a protease or phophatase inhibitor tablet or mini tablet that contains EDTA?

Metalloproteases bind metal ions in their active sites. EDTA is a metalloprotease inhibitor that acts by chelating metal ions that are required by these proteases for activity. We recommend using the protease or phosphatase inhibitor tablet or mini tablet containing EDTA if any metalloprotease is likely to adversely affect the functioning of the target protein. However, it is important to avoid using EDTA when the downstream application involves metal chelation affinity purification or if the target protein requires metals ions for proper function.
Which protease and phosphatase inhibitor tablets do you offer?

The Pierce protease, phosphatase, as well as (protease and phosphatase) inhibitors are offered as mini tablets, with or without EDTA. Each mini tablet is sufficient for 10 mL of solution and is suitable for reagent preparation prior to protein extraction for cultured cells and tissues. In addition, the Pierce protease inhibitor tablets are offered with or without EDTA and each tablet is sufficient for 50 mL of solution.

Here are the catalog numbers:
-Pierce Protease Inhibitor Mini Tablets (Cat. No. A32953)
-Pierce Protease inhibitor Mini Tablets, EDTA-free (Cat. No. A32955)
-Pierce Phosphatase Inhibitor Mini Tablets (Cat. No. A32957)
-Pierce Protease and Phosphatase Inhibitor Mini Tablets (Cat. No. A32959)
-Pierce Protease and Phosphatase Inhibitor Mini Tablets, EDTA-free (Cat. No. A32961)
-Pierce Protease Inhibitor Tablets (Cat. No. A32963)
-Pierce Protease Inhibitor Tablets, EDTA-free (Cat. No. A32965)

For Research Use Only. Not for use in diagnostic procedures.