Please login to your online account to display your discounted pricing

Thermo Scientific™ Pierce™ Premium Grade DSP

Optimize applications where product integrity and risk minimization are paramount with our highest quality formulation of this popular amine-reactive, cleavable crosslinker.

$389.55 - $2,541.00

Formulation Dithiobis(succinimidylpropionate); powder solid
View More Specs
Products
Catalog Number Mfr. No. Formulation Sufficient For Quantity Price Quantity    

PIPG82081

 
thermo scientific™
PG82081
Dithiobis(succinimidylpropionate); powder solid 2.47L of typical 1X (1mM) reaction solution 1g Each for $389.55

PIPG82082

 
thermo scientific™
PG82082
Dithiobis(succinimidylpropionate); powder solid 24.7L of typical 1X (1mM) reaction solution 10g Each for $2,541.00
Description & Specifications

Specifications

Formulation Dithiobis(succinimidylpropionate); powder solid

Optimize applications where product integrity and risk minimization are paramount with the specially characterized Thermo Scientific™ Pierce™ Premium Grade DSP, our highest quality formulation of this popular amine-reactive, cleavable crosslinker.

Thermo Scientific Pierce Premium Grade DSP is our highest quality formulation of this popular amine-reactive, cleavable crosslinker, specially characterized for applications where product integrity and risk minimization are paramount.

Compared to the standard grade product, Premium Grade DSP provides more clearly defined quality and product support by including (a) increased analytical testing and product characterization, (b) greater batch-specific information and quality assurance review, (c) extensive lot sample retention and (d) change control notification. DSP is dithiobis(succinimidyl propionate), an amine-reactive crosslinker having NHS-ester reactive ends and a cleavable disulfide bond in its spacer arm. DSP contains an amine-reactive N-hydroxysuccinimide (NHS) ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxy-succinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS-ester crosslinking reagents.

Highlights:

  • DSP – a thiol-cleavable, homobifunctional and amine-reactive crosslinking agent
  • High quality – identity and purity confirmed by several tests, including quantitative NMR
  • Product integrity – enhanced level of testing and characterization compared to standard grade
  • Lot retention – ample supply of past lots retained to ensure future process testing
  • Change management – Change Control Notification (CCN) service
  • Consistent manufacture – batch-specific manufacturing documentation review

Applications:

  • Chemical crosslinking of intracellular proteins prior to cell lysis and immunoprecipitation
  • “Fix” protein interactions to allow identification of weak or transient protein interactions
  • Fix tissues for immunostaining without damaging RNA
  • Protein crosslinking to create bioconjugates via single-step reactions
  • Immobilize proteins onto amine-coated surfaces