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Thermo Scientific™ Pierce™ Phosphoprotein Enrichment Kit


Purify (enrich) phosphorylated proteins from mammalian cell and tissue lysates using phospho-specific IMAC affinity resin cartidges or kit.

Manufacturer: thermo scientific™  90003**

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Catalog No. PI90003

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Description & Specifications

Specifications

Kit Contents CHAPS Detergent, 1g
Support Type Agarose
Quantity 10 column kit
Description Pierce Phosphoprotein Enrichment Kit, 1mL
Formulation Multi-component kit with 10 x 1mL resin columns
Sufficient For Purifying 10 protein samples each containing up to 4mg total protein (400ug phosphoprotein)
Item Description Kit with 1mL resin columns; 10-column kit

Perform efficient enrichment of phosphorylated proteins derived from mammalian cells and tissues with Thermo Scientific™ Pierce Phosphoprotein Enrichment Kit.

The kit is an effective system for isolating the phosphoprotein fraction of mammalian cell and tissue lysates. The included lysis-binding buffer and proprietary affinity resin produce superior yields with negligible non-specific binding. The kit uses a convenient spin column format, and the resulting phosphorylated protein sample is compatible with downstream applications, including Western blotting, mass spectrometry (MS), 2D-PAGE, and protein arrays. The affinity resin is also available as pre-packed chromatography cartridges for use with automated liquid chromatography (LC) systems.

Highlights:

  • Specific – low nonspecific protein contamination from complex biological samples, such as cell culture lysate and mouse tissue extract

  • Fast – easy spin format enables enrichment of phosphorylated proteins in less than 2 hours

  • Superior yield – achieves higher yields than other commercially available phosphoprotein isolation kits

  • Convenient format – complete kit offering pre-dispensed spin columns, buffers, reagents and ultrafiltration columns for enrichment

  • Compatible – downstream applications include mass spectrometry, Western blotting and 2D-PAGE