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Thermo Scientific™ Pierce™ Phosphoprotein Enrichment Kit

Purify (enrich) phosphorylated proteins from mammalian cell and tissue lysates using phospho-specific IMAC affinity resin cartidges or kit.

Manufacturer: thermo scientific™  90003**

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Catalog No. PI90003

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Description & Specifications


Kit Contents CHAPS Detergent, 1g
Support Type Agarose
Quantity 10 column kit
Description Pierce Phosphoprotein Enrichment Kit, 1mL
Formulation Multi-component kit with 10 x 1mL resin columns
Sufficient For Purifying 10 protein samples each containing up to 4mg total protein (400ug phosphoprotein)

Perform efficient enrichment of phosphorylated proteins derived from mammalian cells and tissues with Thermo Scientific™ Pierce Phosphoprotein Enrichment Kit.

The kit is an effective system for isolating the phosphoprotein fraction of mammalian cell and tissue lysates. The included lysis-binding buffer and proprietary affinity resin produce superior yields with negligible non-specific binding. The kit uses a convenient spin column format, and the resulting phosphorylated protein sample is compatible with downstream applications, including Western blotting, mass spectrometry (MS), 2D-PAGE, and protein arrays. The affinity resin is also available as pre-packed chromatography cartridges for use with automated liquid chromatography (LC) systems.


  • Specific – low nonspecific protein contamination from complex biological samples, such as cell culture lysate and mouse tissue extract

  • Fast – easy spin format enables enrichment of phosphorylated proteins in less than 2 hours

  • Superior yield – achieves higher yields than other commercially available phosphoprotein isolation kits

  • Convenient format – complete kit offering pre-dispensed spin columns, buffers, reagents and ultrafiltration columns for enrichment

  • Compatible – downstream applications include mass spectrometry, Western blotting and 2D-PAGE