Thermo Scientific™ Pierce™ Phosphoprotein Enrichment Kit
Purify (enrich) phosphorylated proteins from mammalian cell and tissue lysates using phospho-specific IMAC affinity resin cartidges or kit.
Manufacturer: Thermo Scientific™ 90003**
Perform efficient enrichment of phosphorylated proteins derived from mammalian cells and tissues with Thermo Scientific™ Pierce Phosphoprotein Enrichment Kit.The kit is an effective system for isolating the phosphoprotein fraction of mammalian cell and tissue lysates. The included lysis-binding buffer and proprietary affinity resin produce superior yields with negligible non-specific binding. The kit uses a convenient spin column format, and the resulting phosphorylated protein sample is compatible with downstream applications, including Western blotting, mass spectrometry (MS), 2D-PAGE, and protein arrays. The affinity resin is also available as pre-packed chromatography cartridges for use with automated liquid chromatography (LC) systems.
Specific – low nonspecific protein contamination from complex biological samples, such as cell culture lysate and mouse tissue extract
Fast – easy spin format enables enrichment of phosphorylated proteins in less than 2 hours
Superior yield – achieves higher yields than other commercially available phosphoprotein isolation kits
Convenient format – complete kit offering pre-dispensed spin columns, buffers, reagents and ultrafiltration columns for enrichment
Compatible – downstream applications include mass spectrometry, Western blotting and 2D-PAGE
|CHAPS Detergent, 1g|
|Multi-component kit with 10 x 1mL resin columns|
|Purifying 10 protein samples each containing up to 4mg total protein (400ug phosphoprotein)|
|Pierce Phosphoprotein Enrichment Kit, 1mL|
|10 column kit|