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  6. Thermo Scientific™ Pierce™ Phosphatase Inhibitor Mini Tablets

Thermo Scientific™ Pierce™ Phosphatase Inhibitor Mini Tablets

Catalog No. PIA32958
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2 Tablets
20 Tablets
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Catalog No. Quantity
PIA32958 2 Tablets
PIA32957 20 Tablets
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For Research Use Only. Not for use in diagnostic procedures.
Catalog No. PIA32958 Supplier Thermo Scientific™ Supplier No. A32958
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For Research Use Only. Not for use in diagnostic procedures.

Description

Thermo Scientific™ Pierce Phosphatase Inhibitor Mini Tablets provide complete cell lysate and biological sample protection from dephosphorylation with a broad spectrum phosphatase inhibitor formulation.

Pierce Phosphatase Inhibitor Mini Tablets protect phosphoproteins from serine/threonine phosphatases and tyrosine phosphatases. Each Phosphatase Inhibitor Tablet contains sodium fluoride, sodium orthovanadate, sodium pyrophosphate and beta-glycerophosphate for broad-spectrum inhibition of phosphatase activity. Tablets are stable at 4°C for up to 12 months, and each tablet is sufficient for 10mL of solution. The Pierce Phosphatase Inhibitor Tablets are suitable for use with any cell or tissue extract to preserve protein phosphorylation for downstream applications.

Highlights:
  • Economical&mdsashmore cost effective than other commercially available formulations
  • Improved formulation&mdsashtablets dissolve quickly into a clear solution
  • Complete&mdsashthe tablets inhibit acid, alkaline, and protein phosphatases
  • Compatible&mdsashuse with homebrew or commercially available cell lysis reagents and Thermo Scientific Protein Assays

Specifications

Includes 1 vial of inhibitor tablets
Content And Storage 2°C to 8°C
For Use With (Application) protein purification, western blotting
Sufficient For 1 Tablet is Sufficient for 10 mL
Quantity 2 Tablets

Frequently Asked Questions (FAQs)

After dissolving the Pierce Phosphatase Inhibitor Mini Tablets (Cat. No. A32957), for how long would the solution be stable at 4 °C?

We have no data on long-term stability of the inhibitors at 4°C in aqueous solution. We generally recommend dissolving the tablets just before use.

Would it be possible to dissolve the Pierce Phosphatase Inhibitor Mini Tablets (Cat. No. A32957) in less than 10 mL in order to have a solution of higer concentration?

As it is recommended to use a final inhibitor concentration of 2X - 3X for samples with a high phosphatase activity, it is indeed possible to prepare a solution at these concentrations. However, we would not recommend to go much higher than 3X.

How should I dissolve Pierce Phosphatase Inhibitor Mini Tablets?

The Pierce Phosphatase Inhibitor Mini Tablets can be dissolved in the lysis reagent that is being used for protein extraction. We recommend dissolving each tablet in 10 mL of the lysis reagent to get a 1X final concentration. To dissolve completely, the solution may be vortexed or placed on a magnetic stir plate with a stir bar.
Can I use just part of the Pierce Protease Inhibitor XL Capsules, EDTA-Free and save part of the capsule for later use?

We do not recommend “splitting” the capsule into smaller portions. Researchers using less than 500 mL should choose a different format (liquid or tablet) of protease inhibitors.
What formats of protease inhibitors do you offer?

We offer standalone protease inhibitors (AEBSF, Aprotinin, PMSF, EDTA, etc.), as well as cocktails or combinations of protease inhibitors in liquid, tablet, and larger capsule formats. These cocktails of protease inhibitors can also be purchased in combination with a cocktail of phosphatase inhibitors, in both liquid and tablet form, for even greater convenience.
Do you offer any of the components of the Pierce protease, phosphatase and (protease and phosphatase) inhibitor tablets and mini tablets individually?

Yes, the following components of the Pierce protease, phosphatase and (protease and phosphatase) inhibitor tablets and mini tablets are offered separately:

- AEBSF (Cat. No. 78431)
- Aprotinin (Cat. No. 78432)
- Bestatin (Cat. No. 8433)
- E-64 (Cat. No. 78434)
- Leupeptin (Cat. No. 78435)
- Pepstatin A (Cat. No. 78436)
- EDTA (Cat. No. 17892).
What are the concentrations of the various components of the protease and phosphatase inhibitor tablets and mini tablets?

The final concentration of each component in the protease and phosphatase inhibitor tablets and mini tablets is proprietary. We release only the identities of the inhibitors.
Can I store the reconstituted solution of protease, phosphatase and (protease and phosphatase) inhibitor tablet or mini tablet and if so, for how long?

The Pierce protease, phosphatase, as well as (protease and phosphatase) inhibitor tablets and mini tablets are stable for at least one year when stored at 4 degrees C, but we only recommend preparing fresh solutions just before needed. If you are interested in liquid protease or phosphatase inhibitors, please use our inhibitor cocktails (https://www.thermofisher.com/search/results?query=protease%20inhibitor%20cocktails&focusarea=Search%20All).
Which protein assay is compatible with the protease, phosphatase, as well as (protease and phosphatase) inhibitor tablets and mini tablets?

The Pierce protease, phosphatase and (protease and phosphatase) inhibitor tablets and mini tablets are compatible with common protein assays such as BCA Protein Assay, 660 nm Protein Assay, Coomassie Plus (Bradford) Assay, and Detergent Compatible Bradford Assay.
When do I need to use a protease or phophatase inhibitor tablet or mini tablet that contains EDTA?

Metalloproteases bind metal ions in their active sites. EDTA is a metalloprotease inhibitor that acts by chelating metal ions that are required by these proteases for activity. We recommend using the protease or phosphatase inhibitor tablet or mini tablet containing EDTA if any metalloprotease is likely to adversely affect the functioning of the target protein. However, it is important to avoid using EDTA when the downstream application involves metal chelation affinity purification or if the target protein requires metals ions for proper function.
Which protease and phosphatase inhibitor tablets do you offer?

The Pierce protease, phosphatase, as well as (protease and phosphatase) inhibitors are offered as mini tablets, with or without EDTA. Each mini tablet is sufficient for 10 mL of solution and is suitable for reagent preparation prior to protein extraction for cultured cells and tissues. In addition, the Pierce protease inhibitor tablets are offered with or without EDTA and each tablet is sufficient for 50 mL of solution.

Here are the catalog numbers:
-Pierce Protease Inhibitor Mini Tablets (Cat. No. A32953)
-Pierce Protease inhibitor Mini Tablets, EDTA-free (Cat. No. A32955)
-Pierce Phosphatase Inhibitor Mini Tablets (Cat. No. A32957)
-Pierce Protease and Phosphatase Inhibitor Mini Tablets (Cat. No. A32959)
-Pierce Protease and Phosphatase Inhibitor Mini Tablets, EDTA-free (Cat. No. A32961)
-Pierce Protease Inhibitor Tablets (Cat. No. A32963)
-Pierce Protease Inhibitor Tablets, EDTA-free (Cat. No. A32965)

For Research Use Only. Not for use in diagnostic procedures.