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Thermo Scientific™ Pierce™ Peptide Retention Time Calibration Mixture

15 synthetic heavy peptides mixed in equimolar ratio to test and calibrate reverse-phase liquid chromatography (LC) and mass spectrometry (LC-MS) equipment.

Manufacturer:  Thermo Scientific™ 88321

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Catalog No. PI88321



Limited warranty period: Subject to all warranty requirements and exclusions set out in our Terms and Conditions of Sale, this product is warranted from the date we ship the product and for ninety (90) days thereafter. Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.



Thermo Scientific™ Pierce Peptide Retention Time Calibration Mixture enables reversed phase liquid chromatography (LC) and mass spectrometry (LC-MS) users to optimize and confirm correct operation of their equipment.

The Pierce Peptide Retention Time Calibration Mixture is used to optimize and assess LC parameters, identify total peptide elution window and optimize MS parameters. Testing the mixture frequently throughout the lifetime of a column or including it in quantitative samples makes it possible to monitor and normalize LC-MS performance between samples and over time. When used with Thermo Scientific Pinpoint 1.1 software, the calibration mixture is used to predict peptide retention times based upon peptide sequence and ease the transfer of methods between LC-MS platforms.


  • Assessment of chromatography and MS instrument performance
  • Prediction of peptide retention across multiple instrument platforms
  • Prediction of peptide retention time from sequence using calculated hydrophobicity factor
  • Optimization of scheduled MS acquisition windows for improved quantification and increased multiplexing
  • Internal standard to normalize for variation in retention times and peak intensities between runs
Description & Specifications


Peptide Retention Time Calibration Mixture
200 to 4000 uses
Mixture of 15 synthetic heavy peptides at 5uM each


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