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Thermo Scientific™ Pierce™ Ni-NTA Magnetic Agarose Beads
Description
Product features:
Ni-NTA Magnetic Agarose Beads are used for small-scale affinity purification as well as high-throughput screening of recombinant His-tagged proteins. The polyhistidine tag is the most popular affinity tag and typically consists of six consecutive histidine residues (6xHis). Tagged proteins are overexpressed in a number of different systems, most commonly in bacteria, and purified from cell lysates such as those prepared using Thermo Scientific B-PER Bacterial Protein Extraction reagents. Purification of His-tagged proteins is achieved using an NTA chelate charged with nickel that coordinates with the histidine side chains. The NTA chelate contains four metal-binding sites that allow for low metal ion leaching and high binding capacity. The protocol for the Ni-NTA Magnetic Agarose Beads has been optimized to allow for high purity of the isolated His-tagged protein.
- High capacity—sufficient for both routine and demanding purification procedures; binds >70 mg of 6xHis-tagged protein per mL of settled resin
- High-performance and reproducible beads—non-aggregating, magnetite (Fe3O4), superparamagnetic beads provide exceptional uniformity for both manual and automated HTS purification applications
- Low nonspecific binding—optimized purification protocol results in better tagged-protein purification
- Versatile—purify proteins using native or denaturing conditions
- Compatible—use with Thermo Scientific Cell Lysis reagents and a variety of buffer additives
Applications:
- Protein purification and isolation
- High throughput protein purification using automated magnetic particle processor
Specifications
Specifications
| Bed Volume | 1 mL |
| Capacity (Metric) | 4 mL |
| Concentration | Slurry: 25% |
| Content And Storage | 1 mL settled resin. Supplied as 4 mL of 25% v/v suspension in 20% ethanol. Store at 2–8°C. |
| Shipping Condition | Wet Ice |
| Particle Size | 30 μm |
| Form | Liquid Suspension |
| Product Line | Pierce |
| Quantity | 4 mL |
| Protein Form | Recombinant |
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Frequently Asked Questions (FAQs)
Yes, 7xHis-tagged proteins, proteins naturally high in histidine, and other combinations of His and other amino acids will bind. To elute them, you have to increase the concentration of imidazole. Generally these peptides will not contaminate your fraction since they remain on the column. However after multiple uses of the same column, these peptides may reduce the binding capacity of the column.
No. Freezing will adversely affect the agarose structure and may cause bead aggregation or loss of binding.
Yes. Protocols for using the beads on a KingFisher instrument are available on the product page.
Pierce Magentic Agarose Beads have a much higher binding capacity.
For Research Use Only. Not for use in diagnostic procedures.