Thermo Scientific™ NHS-Phosphine

Description

Thermo Scientific™ Pierce NHS-Phosphine and Sulfo-NHS-Phosphine are amine-reactive compounds that can be used to derivatize primary amines of proteins or amine-coated polymer surfaces for ligation to azide-tagged molecules.

These NHS-ester compounds react to form covalent bonds with primary amines (e.g., side chain of lysine residues or aminosilane-coated surfaces). The phosphine groups (PPh2) react with azide-labeled molecules by a mechanism known as Staudinger chemistry, enabling efficient and specific conjugation of derivatized molecules in biological samples. In combination with different azide-tagged compounds, these NHS-Phosphine reagents facilitate a variety of labeling and crosslinking experimental strategies for investigating protein interactions and cellular pathways.

Highlights:

• Versatile – Sulfo-NHS-Phosphine easily dissolves in water-miscible solvents for subsequent dilution in aqueous reaction mixtures for azide conjugation; NHS-Phosphine can be used for amine surface modification in organic solvents

• Compatible – reaction chemistry occurs effectively in simple buffer conditions; requires no accessory reagents such as copper or reducing agents

• Specific – NHS esters are specific for covalent attachment to primary amines (side chain of lysine or N-terminus of polypeptides)

• Chemoselective – azide and phosphine groups do not react or interfere with components of biological samples but conjugate to one another with high efficiency