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Thermo Scientific™ Sulfo-SDA (Sulfo-NHS-Diazirine) (sulfosuccinimidyl 4,4'-azipentanoate)

Catalog No. PI26173
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Quantity:
50 mg
Catalog No. Quantity
PI26173 50 mg
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Catalog No. PI26173

Supplier: Thermo Scientific™ LSG26173

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Description

Very short to mid-length (3.9 to 13.5A), amine-to-anything crosslinkers; NHS-ester and diazirine reactive groups; photoactivate with UV light (Amax 330 to 370nm).

Thermo Scientific™ Pierce NHS-Diazirine Crosslinkers combine proven NHS-ester and diazirine-based photoreaction chemistries to conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation.

The succinimidyl-ester diazirine (SDA) reagents are a new class of crosslinkers that combine proven amine-reactive chemistry with an innovative and efficient diazirine-based photochemistry for conjugating amine-containing molecules to nearly any other functional group. The SDA crosslinkers include six compounds differing in spacer arm lengths, ability to cleave the crosslinked proteins, and presence or absence of a charged group for membrane permeability. Protein crosslinking is an important technique used to understand protein structure and to stabilize protein-protein interactions. Thermo Scientific SDA Reagents extend the efficiency and range of interactions that can be explored by this approach.

Highlights:

  • Versatile - different amine-reactive diazirine derivatives capture specific intracellular or cell-surface protein interactions

  • Controllable - two-step chemical crosslinking is activated using common laboratory UV lamps

  • Reversible - disulfide-containing spacers enable cleavage using reducing agents

  • Easy - reagents are photo-stable under typical laboratory lighting conditions so there is no need to perform experiments in the dark

  • Better than aryl azides - diazirine photo-reactive group has better photostability and is more efficiently activated by long-wave UV light

Specifications

Chemical Reactivity Amine-Nonselective
Cleavable No
Description Sulfo-SDA
Molecular Weight (g/mol) 327.25
PEGylated No
Spacer Arm Length 3.9 Å
Content And Storage Upon receipt store product at 4°C protected from light and moisture.
Cell Permeability No
Shipping Condition Ambient
Product Line Pierce
Labeling Method Chemical Labeling
Crosslinker Type Heterobifunctional
Reactive Moiety Diazirine, Sulfo-NHS Ester
Spacer Short (<10 Å)
Form Solid
Quantity 50 mg
Solubility Water
Format Standard
Water Soluble Yes
Product Type Crosslinker
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For Research Use Only. Not for use in diagnostic procedures.