missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Protein Purification
  5. Protein Purification Reagents and Kits
  6. PI26200

Thermo Scientific™ Pierce™ NHS-Activated Agarose Slurry

Catalog No. PI26200
Change view
Click to view available options
Quantity:
25 mL
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
PI26200 25 mL
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. PI26200 Supplier Thermo Scientific™ Supplier No. LSG26200

Description

N-Hydroxysuccinimide ester resin and columns to covalently immobilize proteins, peptides and other amine-containing ligands for affinity purification.

Perform rapid and stable immobilization of proteins, peptides and other ligands with Thermo Scientific™ Pierce NHS-Activated Agarose, a high-quality beaded-agarose support.

The activated affinity resin is available as a slurry or dry powder, allowing simple and efficient immobilization of proteins to a beaded-agarose support. The beaded agarose support contains N-hydroxysuccinimide (NHS) functional groups that react with primary amines to form stable amide linkages. The coupling reaction is performed in an amine-free buffer at pH 7 to 9. Protein coupling efficiency is typically greater than 80%, regardless of the molecular weight or pI the ligand. Coupling capacity exceeds 25mg of protein per milliliter of resin. Once a ligand is immobilized, the prepared resin can be used for multiple affinity purification procedures. The crosslinked beaded agarose has fast linear flow potential, making it useful for gravity-flow and low- to medium-pressure applications.

The Pierce NHS-Activated Agarose resin is available in an anhydrous acetone slurry and as dry powder. The unique dry form does not require storage in or removal and disposal of the acetone solvent. In addition, the dry resin is ideal for coupling reactions with dilute samples because it concentrates the sample as the resin swells, reducing the volume of the starting material and resulting in highly efficient ligand immobilization.

Highlights:

  • Easy to use – immobilize in a simple one-step reaction with minimal hands-on time

  • Rapid and efficient – greater than 85% coupling for most proteins within 30 minutes

  • Innovative – the dry agarose concentrates the sample, making it ideal for immobilizing dilute proteins

  • Safe – no hazardous chemicals needed (e.g., sodium cyanoborohydride, cyanogen bromide)

  • Versatile – affinity resin is adaptable to column and batch affinity chromatography techniques and FPLC applications

  • Compatible – use with any primary amine-containing compound

  • Reusable – the leak-resistant chemistry means you can reuse the affinity resin

  • High binding capacity – binding capacity of >30mg/mL (slurry) or >25mg/mL (dry resin)

Specifications

Capacity (Metric) ≥30 mg per mL Coupling
Content And Storage Upon receipt store the agarose slurry at 4°C. Product is shipped at ambient temperature.
Form Liquid
Product Line Pierce
Quantity 25 mL
Protein Form Recombinant
Target Antibody, Protein, Peptide, Ligand
Type Agarose
Stationary Phase NHS Ester
Format Bottle
Column Type Affinity, Agarose Resin
Show More Show Less

Frequently Asked Questions (FAQs)

Can you provide the shelf-life for NHS-Activated Agarose Slurry?

NHS-Activated Agarose Slurry is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (4 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.
What is the difference between AminoLink Coupling Resin and NHS-Activated Agarose?

Both react with primary amines, but AminoLink Coupling Resin is a two-step conjugation. First a Schiff base is formed between the amine and the aldehyde on the resin, which is then reduced to a stable secondary amine with sodium cyanoborohydride, whereas NHS-Activated Agarose utilizes an NHS ester to form an amide bond in a one-step conjugation.
How does the NHS-activated agarose resin work, and what are its benefits?

NHS- activated agarose reacts with primary and secondary amines to form stable amide and imide linkages respectively. Most proteins can be coupled in 30 mins with greater than 85% coupling efficiency. No hazardous chemicals are used, and the agarose is adaptable to use with spin columns, batch methods, or FPLC. The resin is resusable typically up to 10 purifications with the immobilized ligand, and has a high binding capacity (>30 mg/mL slurry or >25 mg/mL dry resin).

For Research Use Only. Not for use in diagnostic procedures.