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Thermo Scientific™ Pierce™ NeutrAvidin™ Protein and Conjugates

Purified, HRP-, AP- or FITC-conjugated or maleimide-activated NeutrAvidin protein for Western blot, ELISA or other biotin-based detection methods.

Manufacturer: thermo scientific™  31007

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Catalog No. PI31007

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Description & Specifications


Sufficient For Conjugation to sulfhydryl groups of approx. 5mg of antibody (150kDa) or molar equivalent
Description Pierce NeutrAvidin Protein, Maleimide Activated
Formulation Lyophilized powder from activated protein in buffered saline solution
Quantity 5mg

Thermo Scientific™ NeutrAvidin Protein is a specially-prepared form of avidin biotin-binding protein that decreases background in Western blotting and ELISA; available items include standard HRP, AP and fluorescent conjugates for biotin-based assays.

NeutrAvidin Protein is deglycosylated native avidin from egg whites. Removal of the excess carbohydrate by an exclusive process yields a protein with a more neutral isoelectric point and less nonspecific binding properties. Purified and conjugated forms of NeutrAvidin Protein provide exceptional performance in Western blot, ELISA and IHC applications that require biotin-binding probes. Assay specificity, sensitivity, and signal-to-noise ratios with NeutrAvidin Protein are generally equivalent or better than with the significantly more expensive streptavidin.


  • Near-neutral isoelectric point – pI equals 6.3, more neutral than native avidin

  • Nearly devoid of glycosylation – decreased possibility of lectin binding compared to native avidin

  • No RYD recognition sequence – no known off-target binding domains like streptavidin

  • Affordable – significantly less expensive than streptavidin

  • Available in multiple formats – choose purified NeutrAvidin Protein, prepared HRP and AP conjugates or activated forms for a variety of techniques