Thermo Scientific™ Pierce™ NeutrAvidin™ Protein and Conjugates
Purified, HRP-, AP- or FITC-conjugated or maleimide-activated NeutrAvidin protein for Western blot, ELISA or other biotin-based detection methods.
Manufacturer: Thermo Scientific™ 31007
Thermo Scientific™ NeutrAvidin Protein is a specially-prepared form of avidin biotin-binding protein that decreases background in Western blotting and ELISA; available items include standard HRP, AP and fluorescent conjugates for biotin-based assays.NeutrAvidin Protein is deglycosylated native avidin from egg whites. Removal of the excess carbohydrate by an exclusive process yields a protein with a more neutral isoelectric point and less nonspecific binding properties. Purified and conjugated forms of NeutrAvidin Protein provide exceptional performance in Western blot, ELISA and IHC applications that require biotin-binding probes. Assay specificity, sensitivity, and signal-to-noise ratios with NeutrAvidin Protein are generally equivalent or better than with the significantly more expensive streptavidin.
Near-neutral isoelectric point – pI equals 6.3, more neutral than native avidin
Nearly devoid of glycosylation – decreased possibility of lectin binding compared to native avidin
No RYD recognition sequence – no known off-target binding domains like streptavidin
Affordable – significantly less expensive than streptavidin
Available in multiple formats – choose purified NeutrAvidin Protein, prepared HRP and AP conjugates or activated forms for a variety of techniques
|Conjugation to sulfhydryl groups of approx. 5mg of antibody (150kDa) or molar equivalent|
|Pierce NeutrAvidin Protein, Maleimide Activated|
|Lyophilized powder from activated protein in buffered saline solution|