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Thermo Scientific™ Pierce™ Mouse IgG1 Fab and F(ab')2 Preparation Kit

Catalog No. PI44980
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2.5 mL kit
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Catalog No. Quantity
PI44980 2.5 mL kit
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Catalog No. PI44980 Supplier Thermo Scientific™ Supplier No. LSG44980
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Description

Efficiently digest mouse IgG1 antibodies into Fab or F(ab')2 fragments with immobilized ficin protease.

The Thermo Scientific Pierce Mouse IgG1 Fab and F(ab')2 Preparation Kit uses immobilized ficin protease to efficiently digest mouse IgG1 into Fab or F(ab')2 fragments, depending on the chosen reaction conditions.

Features of the Mouse IgG1 Fab and F(ab')2 Preparation Kit:

  • High capacity—kit provides for fragmenting 0.25 to 4 mg IgG
  • Complete—kit includes all reagents needed to prepare and purify antibody fragments
  • Enzyme-free digestion products—Immobilized Ficin (beaded agarose resin) provides for control of the digestion reaction and complete removal of resulting antibody fragments from the proteolytic enzyme
  • Optimized for mouse IgG1—the kit procedure is optimized for mouse IgG1; the only effective method for this IgG species and subclass

Related Products

  • Pierce™ Mouse IgG1 Fab and F(ab')2 Micro Preparation Kit (Cat. No. 44680)
  • Pierce™ F(ab')2 Preparation Kit (Cat. No. 44988)
  • Pierce™ Fab Preparation Kit (Cat. No. 44985)

Specifications

Product Line Pierce
Quantity 2.5 mL kit
Kit Contents Pierce Mouse IgG1 Fab and F(ab')2 Preparation Kit
Content And Storage Sufficient For: 10 antibody samples, each containing 0.25 to 4 mg IgG
• Pierce Ficin Agarose, 2.5 mL
• Cysteine-HCl, 1 g
• Mouse IgG1 Digestion Buffer, 120 mL
• NAb Protein A Plus Spin Column, 1 mL, 1 column
• Protein A Binding Buffer, 120 mL
• IgG Elution Buffer, 120 mL
• Zeba Desalt Spin Columns, 7K MWCO, 2 mL, 10 columns
• Spin Columns, 0.8 mL, 10 columns
• Microcentrifuge tubes, 2 mL, 30 tubes

Store at 4–8°C.

For Research Use Only. Not for use in diagnostic procedures.