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Thermo Scientific™ Pierce™ Mouse IgG1 Fab and F(ab')2 Micro Preparation Kit

Catalog No. PI44680
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10 Antibody Samples
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Catalog No. Quantity
PI44680 10 Antibody Samples
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Catalog No. PI44680 Supplier Thermo Scientific™ Supplier No. 44680
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Description

Efficiently digest mouse IgG1 antibodies into Fab or F(ab')2 fragments with immobilized ficin protease.

The Thermo Scientific Pierce Mouse IgG1 Fab and F(ab')2 Micro Preparation Kit uses immobilized ficin protease to efficiently digest mouse IgG1 into Fab or F(ab')2 fragments, depending on the chosen reaction conditions.

Features of the Mouse IgG1 Fab and F(ab')2 Micro Preparation Kit:

  • Capacity—kit provides for fragmenting 25 to 250 μg IgG
  • Complete—kit includes all reagents needed to prepare and purify antibody fragments
  • Enzyme-free digestion products—Immobilized Ficin (beaded agarose resin) provides for control of the digestion reaction and complete removal of resulting antibody fragments from the proteolytic enzyme
  • Optimized for mouse IgG1—the kit procedure is optimized for mouse IgG1; the only effective method for this IgG species and subclass

Related Products

  • Pierce™ Mouse IgG1 Fab and F(ab')2 Preparation Kit (Cat. No. 44980)
  • Pierce™ F(ab')2 Micro Preparation Kit (Cat. No. 44688)
  • Pierce™ Fab Micro Preparation Kit (Cat. No. 44685)

Specifications

Product Line Pierce
Quantity 10 Antibody Samples
Kit Contents Pierce Mouse IgG1 Fab and F(ab')2 Micro Preparation Kit
Content And Storage Sufficient For: 10 antibody samples, each containing 25 to 250 μg IgG
• Pierce Ficin Agarose, 0.8 mL
• Cysteine-HCl, 1 g
• Mouse IgG1 Digestion Buffer, 120 mL
• NAb Protein A Plus Spin Columns, 0.2 mL, 2 columns
• Protein A Binding Buffer, 50 mL
• IgG Elution Buffer, 50 mL
• Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, 10 columns
• Spin Columns, 0.8 mL, 10 columns
• Microcentrifuge tubes, 2 mL, 30 tubes

Store at 4–8°C.

For Research Use Only. Not for use in diagnostic procedures.