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  6. Thermo Scientific™ Pierce™ High Capacity Protein A MagBeads, alkali stable

Thermo Scientific™ Pierce™ High Capacity Protein A MagBeads, alkali stable

Numéro de catalogue. PIA53038
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4 x 25 mL
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PIA53038 4 x 25 mL
PIA53035 1 mL
PIA53036 5 mL
PIA53037 25 mL
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Numéro de catalogue. PIA53038 Fournisseur Thermo Scientific™ Code fournisseur A53038

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Description

The Pierce High-Capacity Protein A MagBeads, Alkali Stable are a high-performance magnetic bead for purifying antibodies from cell culture supernatant, ascites, serum, and crude extracts.

Pierce High-Capacity Protein A MagBeads, Alkali Stable, are high-performance magnetic beads for purification of antibodies from cell culture supernatant, ascites, serum, and crude extracts.

Features of the High-Capacity Protein A MagBeads include:

  • High binding capacity—≥ 40 mg HIgG/mL settled beads
  • Alkaline stable—engineered to withstand standard CIP procedures with 0.1 M NaOH, enabling efficient cleaning of the beads for reuse
  • Animal component-free raw materials
  • Versatile—beads are compatible with manual and automated workflows (e.g., KingFisher instruments)
  • Stable—high-affinity Protein A is securely immobilized to magnetic agarose to help prevent leaching of ligand, target antibody, or base support

The protein A ligand has been genetically modified for stability in the presence of NaOH, so the beads can be regenerated at least 10 times without sacrificing binding capacity. The ligand is produced in yeast and is immobilized on magnetic agarose with an average particle size of ∼30 μm.

Pierce High-Capacity Protein A MagBeads, Alkali-Stable, are suitable for purifying mAbs and a subpopulation of scFv's and Fab fragments containing a VH3 domain. The beads are compatible with automation, allowing for higher throughput purifications and screening of purification conditions.

Spécifications

Bed Volume 25 mL
Binding Capacity ≥40 mg HIgG/mL settled beads
Capacity (Metric) 4 x 25 mL
Concentration Slurry: 25%
Description High Capacity Magnetic Agarose Beads
Content And Storage Provided in the form of a 25% slurry.

Upon receipt store at 2–8°C.
Particle Size 25 to 30 μm
Quantity 4 x 25 mL
For Use With (Application) Antibody Purification
Ligand Type Alkaline Stable Protein A
Format Bottle
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For Research Use Only. Not for use in diagnostic procedures.