missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Protein Purification
  5. Magnetic Affinity Separation Particles
  6. Thermo Scientific™ Pierce™ High Capacity Protein A MagBeads, alkali stable

Thermo Scientific™ Pierce™ High Capacity Protein A MagBeads, alkali stable

Catalog No. PIA53038
Change view
Click to view available options
Quantity:
1 mL
5 mL
25 mL
4 x 25 mL
4 product options available for selection
Product selection table with 4 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
PIA53038 4 x 25 mL
PIA53035 1 mL
PIA53036 5 mL
PIA53037 25 mL
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 4 options available.
4 options
Catalog No. PIA53038 Supplier Thermo Scientific™ Supplier No. A53038

Description

The Pierce High-Capacity Protein A MagBeads, Alkali Stable are a high-performance magnetic bead for purifying antibodies from cell culture supernatant, ascites, serum, and crude extracts.

Pierce High-Capacity Protein A MagBeads, Alkali Stable, are high-performance magnetic beads for purification of antibodies from cell culture supernatant, ascites, serum, and crude extracts.

Features of the High-Capacity Protein A MagBeads include:

  • High binding capacity—≥ 40 mg HIgG/mL settled beads
  • Alkaline stable—engineered to withstand standard CIP procedures with 0.1 M NaOH, enabling efficient cleaning of the beads for reuse
  • Animal component-free raw materials
  • Versatile—beads are compatible with manual and automated workflows (e.g., KingFisher instruments)
  • Stable—high-affinity Protein A is securely immobilized to magnetic agarose to help prevent leaching of ligand, target antibody, or base support

The protein A ligand has been genetically modified for stability in the presence of NaOH, so the beads can be regenerated at least 10 times without sacrificing binding capacity. The ligand is produced in yeast and is immobilized on magnetic agarose with an average particle size of ∼30 μm.

Pierce High-Capacity Protein A MagBeads, Alkali-Stable, are suitable for purifying mAbs and a subpopulation of scFv's and Fab fragments containing a VH3 domain. The beads are compatible with automation, allowing for higher throughput purifications and screening of purification conditions.

Specifications

Bed Volume 25 mL
Binding Capacity ≥40 mg HIgG/mL settled beads
Capacity (Metric) 4 x 25 mL
Concentration Slurry: 25%
Description High Capacity Magnetic Agarose Beads
Content And Storage Provided in the form of a 25% slurry.

Upon receipt store at 2–8°C.
Particle Size 25 to 30 μm
Quantity 4 x 25 mL
For Use With (Application) Antibody Purification
Ligand Type Alkaline Stable Protein A
Format Bottle
Show More Show Less

Frequently Asked Questions (FAQs)

Can I automate the workflow using Pierce High Capacity Protein A MagBeads, alkali stable?

Yes. A KingFisher Apex protocol for 96-deep well plates is provided on pages 3 and 4 in the instruction manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0026311_Pierce_HiCap_proteinA_MagBeads_UG.pdf).
Using Pierce High Capacity Protein A MagBeads, alkali stable, what downstream assays are compatible with the eluted samples?

Purified samples eluted from Pierce High Capacity Protein A MagBeads, alkali stable are compatible with protein assays (Bradford, BCA, Rapid Gold, A280, etc.) and can be used for industry standard testing applications (western blotting, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, etc.).
After regeneration of Pierce High Capacity Protein A MagBeads, alkali stable, is there a specific buffer in which the beads should be stored?

If the beads are not going to be re-used immediately, we recommend using 20% ethanol for long-term storage.
How do I regenerate Pierce High Capacity Protein A MagBeads, alkali stable?

Using the Clean-In-Place (CIP) protocol provided in the instruction manual, we recommend cleaning the Pierce High Capacity Protein A MagBeads, alkali stable with 20 bed volumes of 0.1 M NaOH after every run.
How many uses/regenerations are possible with Pierce High Capacity Protein A MagBeads, alkali stable?

Pierce High Capacity Protein A MagBeads, alkali stable can be reused at least 10 times with <5% loss in binding capacity.

For Research Use Only. Not for use in diagnostic procedures.