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Thermo Scientific™ Pierce™ Glutathione Superflow Agarose

Catalog No. PI25239
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PI25239 1 L
PI25237 50 mL
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Catalog No. PI25239 Supplier Thermo Scientific™ Supplier No. LSG25239
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Description

Perform high-purity, large-scale purification of GST-tagged proteins with glutathione that has been immobilized to a crosslinked 6% beaded agarose.

Perform high-purity, large-scale purification of GST-tagged proteins with Thermo Scientific™ Pierce Glutathione Superflow Agarose, which consists of glutathione that has been immobilized to a crosslinked 6% beaded agarose.

Additional Information and Online Ordering

Pierce Glutathione Superflow Agarose is a highly crosslinked, durable resin that does not compress at flow rates required for medium- to large-scale FPLC purifications. The resin holds up well to a variety of chemical and pH values, and it is compatible with common clean-in-place procedures. The specific binding of glutathione to the GST-tag results in high purity and high yield in a single step purification process.

Highlights:

  • High dynamic binding capacity – binds 10mg of pure GST per mL of resin at a linear flow rate of 150cm/hour; can bind greater than 20mg/mL at 30cm/hour flow rate

  • High purity – provides greater than 90% purity when used to purify overexpressed GST or GST-fusion proteins from lysates

  • Robust – highly crosslinked 6% agarose Superflow beaded support tolerates linear flow rates up to 1200cm/hour

  • Compatible – maintains function after exposure to a wide variety of chemicals and pH values

  • Cost effective – resin is stable through multiple cycles of cleaning and reuse

Recommended for:

Medium- to large-scale FPLC purification of GST-tagged proteins

Specifications

Description Pierce Glutathione Superflow Agarose
Formulation Glutathione onhighly crosslinked 6% beaded agarose (superflow 6), slurried in water with sodium azide
Quantity 1 L
Sufficient For Binding up to 30mg GST/mL resin (static capacity) or up to 10mg GST/mL resin (dynamic capacity)

For Research Use Only. Not for use in diagnostic procedures.