Thermo Scientific™ Pierce™ ConA and WGA Glycoprotein Isolation Kits
Purify glycoproteins from serum and cell lysates using affinity resins or kits with concanavilin A (ConA) or wheat germ agglutinin (WGA) lectins.
Manufacturer: Thermo Scientific™ 89805
Thermo Scientific™ Pierce Glycoprotein Isolation Kits use ConA and WGA lectin affinity resins and optimized buffers to efficiently isolate glycoproteins from serum, tissue and cultured cell lysates, thus enabling downstream analysis.These two kits use lectins concanavilin A (ConA) and wheat germ agglutinin (WGA), respectively, as ligands to bind specific classes of glycoproteins from complex protein samples. ConA lectin recognizes alpha-linked mannose and terminal glucose residues, while WGA lectin selectively binds to N-Acetyl glucosamine (GlcNAc) groups and to sialic acid. The kits include ConA or WGA beaded agarose resin, optimized binding/wash and elution buffers, and easy-to-use microcentrifuge spin columns. The kits have been used to successfully isolate glycoproteins from serum as well as HeLa and CHO cell lysates in approximately 50 minutes.
Glycosylation is a post-translational modification that plays an important role in biological functions, including immune regulation, inflammation, cell-to-cell adhesion and cell signaling. Asparagine (N-linked) and serine/threonine residues (O-linked) are glycosylated during passage through the endoplasmic reticulum and golgi apparatus in eukaryotic and prokaryotic (i.e., Archaea and Eubacteria) systems. Glycoconjugates are important for immune regulation, inflammation, cell-to-cell adhesion and contact inhibition, cell signaling, protection against proteolytic degradation, and other biological processes.
High recovery – equivalent or greater glycoprotein recovery than the kits and lectin resins of other suppliers
Fast – glycoprotein purification in less than one hour
Versatile – isolate glycoproteins from various sample types; e.g., human serum and cell lysate
Robust – lectin does not leach from resin when processing sample
Convenient – complete kits include lectin resins and spin columns with all necessary reagents
Compatible with Bradford-based protein assays – dialysis or protein precipitation of recovered glycoproteins is not required prior to protein assay
ConA or WGA beaded agarose resin (1.1mL), binding/wash buffer, elution buffer, and microcentrifuge spin columns and accessories.
|10 rxn kit|
|Binding/Wash Buffer (5X), 6.5mL|
|Pierce Glycoprotein Isolation Kit, WGA|
|Multi-component kit with wheat germ agglutinin agarose resin|
|Isolating glycoproteins with strong affinity for WGA from 10 samples of up to 640uL (1 to 1.5mg total protein) each|