Thermo Scientific™ Pierce™ Glycoprotein Isolation Kit, WGA

Description

Thermo Scientific™ Pierce Glycoprotein Isolation Kits use ConA and WGA lectin affinity resins and optimized buffers to efficiently isolate glycoproteins from serum, tissue and cultured cell lysates, thus enabling downstream analysis.

Glycosylation is a post-translational modification that plays an important role in biological functions, including immune regulation, inflammation, cell-to-cell adhesion and cell signaling. Asparagine (N-linked) and serine/threonine residues (O-linked) are glycosylated during passage through the endoplasmic reticulum and golgi apparatus in eukaryotic and prokaryotic (i.e., Archaea and Eubacteria) systems. Glycoconjugates are important for immune regulation, inflammation, cell-to-cell adhesion and contact inhibition, cell signaling, protection against proteolytic degradation, and other biological processes.

Highlights:

• High recovery – equivalent or greater glycoprotein recovery than the kits and lectin resins of other suppliers

• Fast – glycoprotein purification in less than one hour

• Versatile – isolate glycoproteins from various sample types; e.g., human serum and cell lysate

• Robust – lectin does not leach from resin when processing sample

• Convenient – complete kits include lectin resins and spin columns with all necessary reagents

• Compatible with Bradford-based protein assays – dialysis or protein precipitation of recovered glycoproteins is not required prior to protein assay

Includes:

ConA or WGA beaded agarose resin (1.1mL), binding/wash buffer, elution buffer, and microcentrifuge spin columns and accessories.