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Thermo Scientific™ Chemiluminescent Nucleic Acid Detection Module Kit

Detect biotin-labeled nucleic acid probes in various membrane blotting applications with this complete chemiluminescent detection system.

Manufacturer:  Thermo Scientific™ 0089880

Catalog No. PI89880



Description

Description

Thermo Scientific™ Pierce Chemiluminescent Nucleic Acid Detection Module is a complete system for detection of biotin-labeled nucleic acid probes in various membrane blotting applications.

The Thermo Scientific Chemiluminescent Nucleic Acid Detection Module is a complete system for detection of biotin-labeled nucleic acids for various blotting applications including Northern/Southern blots, ribonuclease protection assays (RPAs) and electrophoretic mobility shift assays (EMSAs). This module provides the essential detection components used in the Thermo Scientific North2South and LightShift EMSA Kits. The system includes an enhanced luminol substrate for horseradish peroxidase (HRP) with optimized blocking and wash buffers that together produce sensitivity equivalent to radioactive (32P) systems. Obtain publication-quality blots having excellent signal and low background.

Highlights:

  • Fast and sensitive – achieve the same speed and detection levels you've come to expect with the North2South and LightShift Kits

  • Versatile – kit protocol (included) is applicable to any biotin-based nucleic acid detection system: Northerns, Southerns, gel shifts, RPAs and colony lifts

  • Flexible – kit contains only the critical detection components for those who already have biotin-labeled probes and an optimized hybridization method developed

Includes:

Stabilized Streptavidin-HRP Conjugate 1.5mL, Luminol/Enhancer Solution 80mL, Stable Peroxide Solution 80mL, Blocking Buffer 500mL, 4X Wash Buffer 500mL, Substrate Equilibration Buffer 500mL

Specifications

Specifications

Kit
10 Blots
Pierce™
Chemiluminescent
Nucleic Acid Detection Module Kit
Sufficient For: 800 cm2 of membrane (10 mini-gel blots)
• Stabilized Streptavidin-HRP, 1.5 mL
• Luminol/Enhancer Solution, 80 mL
• Stable Peroxide Solution, 80 mL
• Blocking Buffer (Part No. 89880A), 500 mL
• Wash Buffer (4X), 500 mL
• Substrate Equilibration Buffer, 500 mL

Store at 4°C.
SDS
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