missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Molecular Biology Reagents and Kits
  4. Nucleic Acid Labeling and Detection
  5. PI89880

Thermo Scientific™ Chemiluminescent Nucleic Acid Detection Module Kit

Catalog No. PI89880
Change view
Click to view available options
Quantity:
10 Blots
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
PI89880 10 Blots
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. PI89880 Supplier Thermo Scientific™ Supplier No. 89880
Only null left

Description

Detect biotin-labeled nucleic acid probes in various membrane blotting applications with this complete chemiluminescent detection system.

Thermo Scientific™ Pierce Chemiluminescent Nucleic Acid Detection Module is a complete system for detection of biotin-labeled nucleic acid probes in various membrane blotting applications.

The Thermo Scientific Chemiluminescent Nucleic Acid Detection Module is a complete system for detection of biotin-labeled nucleic acids for various blotting applications including Northern/Southern blots, ribonuclease protection assays (RPAs) and electrophoretic mobility shift assays (EMSAs). This module provides the essential detection components used in the Thermo Scientific North2South and LightShift EMSA Kits. The system includes an enhanced luminol substrate for horseradish peroxidase (HRP) with optimized blocking and wash buffers that together produce sensitivity equivalent to radioactive (32P) systems. Obtain publication-quality blots having excellent signal and low background.

Highlights:

  • Fast and sensitive – achieve the same speed and detection levels you've come to expect with the North2South and LightShift Kits

  • Versatile – kit protocol (included) is applicable to any biotin-based nucleic acid detection system: Northerns, Southerns, gel shifts, RPAs and colony lifts

  • Flexible – kit contains only the critical detection components for those who already have biotin-labeled probes and an optimized hybridization method developed

Includes:

Stabilized Streptavidin-HRP Conjugate 1.5mL, Luminol/Enhancer Solution 80mL, Stable Peroxide Solution 80mL, Blocking Buffer 500mL, 4X Wash Buffer 500mL, Substrate Equilibration Buffer 500mL

Specifications

Content And Storage Sufficient For: 800 cm2 of membrane (10 mini-gel blots)
• Stabilized Streptavidin-HRP, 1.5 mL
• Luminol/Enhancer Solution, 80 mL
• Stable Peroxide Solution, 80 mL
• Blocking Buffer (Part No. 89880A), 500 mL
• Wash Buffer (4X), 500 mL
• Substrate Equilibration Buffer, 500 mL

Store at 4°C.
Detection Method Chemiluminescence
Format Kit
Product Line Pierce
Product Type Nucleic Acid Detection Module Kit
Quantity 10 Blots

Frequently Asked Questions (FAQs)

Will SuperSignal West Pico PLUS Chemiluminescent Substrate work with nucleic acid blots (Southern blotting, etc.)?

Yes. However, SuperSignal West Pico PLUS Chemiluminescent Substrate was optimized for use in Western blots and is generally not sensitive enough for most nucleic acid protocols. For Southern and Northern blotting, use our North2South Chemiluminescent Nucleic Acid Hybridization and Detection Kit (Cat. No. 17097), North2South Chemiluminescent Substrate for HRP (Cat. No. 17295), or the Chemiluminescent Nucleic Acid Detection Kit, if probing for biotinylated probes (Cat. No. 89880).
Will SuperSignal West Femto Maximum Sensitivity Substrate work with nucleic acid blots (Southern blotting, etc.)?

Yes. However, SuperSignal West Femto Maximum Sensitivity Substrate was optimized for use in Western blot sand is generally not sensitive enough for most nucleic acid protocols. For Southern and Northern blotting, use our North2South Chemiluminescent Nucleic Acid Hybridization and Detection Kit (Cat. No. 17097), North2South Chemiluminescent Substrate for HRP (Cat. No. 17295), or the Chemiluminescent Nucleic Acid Detection Kit, if probing for biotinylated probes (Cat. No. 89880).
Will SuperSignal West Dura Extended Duration Substrate work with nucleic acid blots (Southern blotting, etc.)?

Yes. However, SuperSignal West Dura Extended Duration Substrate was optimized for use in Western blots and is generally not sensitive enough for most nucleic acid protocols. For Southern and Northern blotting, use our North2South Chemiluminescent Nucleic Acid Hybridization and Detection Kit (Cat. No. 17097), North2South Chemiluminescent Substrate for HRP (Cat. No. 17295), or the Chemiluminescent Nucleic Acid Detection Kit, if probing for biotinylated probes (Cat. No. 89880).

For Research Use Only. Not for use in diagnostic procedures.