Perform immunoprecipitation of recombinant HA-tagged proteins expressed in bacterial or mammalian cells or in vitro systems, using manual or robotic magnetic separators, with Thermo Scientific™ Pierce™ Anti-HA Magnetic Beads.
The blocked magnetic bead surface is coated with anti–HA antibody, a highly specific mouse IgG1 monoclonal antibody that recognizes the HA-epitope tag (YPYDVPDYA) derived from the human influenza hemagglutinin (HA) protein. The Pierce Anti-HA Magnetic Beads can be used manually with a magnetic stand, as well as with automated platforms, such as the Thermo Scientific™ KingFisher Instruments, for high-throughput workflows.
Specific – highly specific anti–HA monoclonal antibody enables high–yield and high-purity immunoprecipitation (IP)
Fast – optimized protocol is completed in approximately 1 hour