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  5. Thermo Scientific™ L-Lysine-2HCl, 4,4,5,5-D4 for SILAC

Thermo Scientific™ L-Lysine-2HCl, 4,4,5,5-D4 for SILAC

Catalog No. PI88438
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Catalog No. Quantity
PI88438 500 mg
PI88437 50 mg
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Catalog No. PI88438 Supplier Thermo Scientific™ Supplier No. 88438

Description

Study protein expression by mass spectrometry with mass variants of lysine, arginine and other amino acids to perform stable isotope labeling with amino acids in cell culture.

Thermo Scientific™ Heavy and Light Amino Acids are used to specifically analyze protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC) quantification kits.

SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of 'heavy' 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.

Highlights:

  • Efficient – 100% label incorporation into proteins of living cells
  • Flexible – different isotopes of heavy and/or light amino acids for arginine, lysine, leucine and proline enable the quantitation of peptides derived from MS-grade proteases
  • Multiplex capabilities – several alternative isotopes of arginine and lysine are available that allow the analysis of multiple treatment conditions in each experiment
  • High-quality supplements – heavy amino acids with >99% isotope purity

Specifications

For Use With (Equipment) Mass Spectrometer
Product Type Amino Acids
Content And Storage Store at room temperature.
Label or Dye Mass Isotope of Amino Acid
Shipping Condition Room Temperature
Starting Material Cells
Final Product Type Protein
Detection Method Mass Spectrometry
Workflow Step Protein Labeling
Quantity 500 mg
Form Solid
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Frequently Asked Questions (FAQs)

Do you offer any heavy amino acids other than the ones in the SILAC Protein Quantitation Kits?

We offer additional isotopes of lysine, including 4,4,5,5 D4 L-Lysine-2HCl (Cat. No. 88437) and 13C6 15N2 L-Lysine-2HCl (Cat. No. 88209), which can be used for multiplexed experiments. 13C6 L-Arginine-HCl (Cat. No. 88210) and 13C6 15N2 L-Arginine-HCl (Cat. No. 89990) are also available to enhance peptide isotope label coverage of tryptic peptides. In addition to lysine and arginine, 13C6 L-Leucine (Cat. No. 88435) can also be used with our powdered SILAC DMEM (Cat. No. 88425) and RPMI-1640 media (Cat. No. 88426). Please check the product page for a current listing.

For Research Use Only. Not for use in diagnostic procedures.