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Thermo Scientific™ Pierce™ Agarose ChIP Kit

Chromatin immunoprecipitation (chromatin IP, ChIP) assay kit containing all reagents, agarose affinity beads, and a simple, fast, reliable protocol.

Manufacturer:  Thermo Scientific™ PI26158

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Catalog No. PI26158



Description

Description

Thermo Scientific™ Pierce Agarose ChIP Kit provides a complete set of reagents and a simple, fast and reproducible protocol to perform chromatin immunoprecipitation (ChIP) assays.

This chromatin IP kit contains sufficient reagents to perform 30 ChIP assays with appropriate controls using an optimized protocol and a convenient spin-column format. The kit includes a complete Chromatin Prep Module (also available separately) to efficiently extract and solubilize crosslinked DNA-protein interaction complexes from cells, plus an immunoprecipitation module that contains high-capacity, ChIP-grade Protein A/G agarose.

Highlights:

  • Simple and streamlined ChIP assay protocol (< 8 hours)
  • Highly efficient isolation and lysis of nuclei
  • Easy and reproducible enzymatic digestion
  • Low-background and high-capacity Protein A/G agarose resin
  • Highly specific positive control reagents included: RNA polymerase II antibody and GAPDH PCR primers
  • Fast and reproducible spin-column format; columns and collection tubes included
  • High-recovery DNA purification
  • Choose a complete ChIP assay kit or the separate chromatin preparation module

Includes:

Kit contains the Chromatin Prep Module (Part No. 26158; 11 components) for chromatin extraction and a complete set of immunoprecipitation reagents (15 components) for IP experiments with controls

Specifications

Specifications

Pierce Chromatin Prep Module
Nuclear Extraction Buffer, 10mL, MNase Digestion Buffer, 5mL, Halt Protease and Phosphatase Inhibitor Cocktail EDTA-free (100X), 4 x 90uL, Glycine Solution (10X), 15mL, DTT, Lyophilized, 1 vial, Micrococcal Nuclease (ChIP Grade) (10 U/µL), MNase Stop Solution, 0.5mL, PBS (20X), 15mL, Proteinase K (20mg/mL), 0.25mL, Sodium Chloride (5M), 3mL, Membrane Extraction Buffer, 10mL
30 chromatin preparations for ChIP assays
Multi-component kit
30 rxn kit
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