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Thermo Scientific™ Pierce™ 3x DYKDDDDK Peptide
Tricosa-peptide for competitively eluting DYKDDDK-tagged fusion proteins from immobilized supports
Supplier: Thermo Scientific™ A36806
Description
Pierce 3X DYKDDDDK Peptide is a lyophilized peptide with the sequence Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys (MDYKDHDGDYKDHDIDYKDDDDK). It has a molecular weight of 2861.9 g/mol. For use, reconstitute to 1.5 mg/mL in PBS.
This tricosa-peptide can be used to competitively elute DYKDDDDK-tagged fusion proteins from immobilized anti-DYKDDDDK affinity supports, especially when a DYKDDDDK-tagged protein is sensitive to low pH. At a 1.5 mg/mL concentration, complete release of tagged protein can be achieved in 5–10 minutes at ambient temperature.
Note: Pierce 3X DYKDDDDK Peptide may interfere with protein determination assays and absorbance at 280 nm. However, the peptide can be removed from the eluate by desalting (e.g., with Zeba Spin Desalting Columns, Cat. No. 89891).
Specifications
| Pierce | |
| Recombinant | |
| 5 x 5 mg lyophilized peptide. Store at -5 to -30°C. | |
| Wet Ice | |
| Lyophilized Powder |
| Peptides | |
| 5 x 5 mg | |
| Lyophilized Powder | |
| Protein Purification and Isolation | |
| Affinity Resin |
For Research Use Only. Not for use in diagnostic procedures.