Thermo Scientific™ Phusion™ Plus DNA Polymerase

Description

Thermo Scientific Phusion Plus DNA Polymerase is a hot-start, high-fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing. It is a Pyrococcus-like proofreading DNA polymerase fused to a processivity-enhancing domain, enabling Phusion Plus DNA Polymerase to generate PCR sequences with high accuracy, sensitivity, and inhibitor tolerance.

Features include:

• >100X higher sequence accuracy than Taq polymerase
• Universal annealing temperature of 60°C in PCR
• Hot-start mechanism reduces non-specific amplification and primer degradation
• Automation enabled due to 24-hour stability of assembled reactions
• High inhibitor tolerance for work with DNA of suboptimal purity

The novel Phusion Plus reaction buffer includes proprietary additives that stabilize primer/template duplexes during the annealing step at 60°C. This unique buffer formulation increases PCR specificity without the need to optimize annealing temperature for each primer pair, helping save time and avoid mistakes in PCR runs. Furthermore, with Phusion Plus DNA Polymerase and its universal annealing feature, different PCR reactions can be cycled together using the same protocol.

Phusion Plus DNA Polymerase is inactive at ambient temperatures due to its hot-start mechanism mediated by Affibody molecules, allowing setup and storage of assembled PCR reactions at room temperature. Enzyme activity is fully restored after the initial denaturation step.

Applications

• High-fidelity PCR
• Cloning & mutagenesis
• Template generation for sequencing
• GC-rich PCR
• Long-range PCR (up to 20 kb)
• High-throughput PCR

Notes

• To simplify reactrion setup, Phusion Plus DNA Polymerase is also available in ready-to-use master mix formats.