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Thermo Scientific™ Phusion™ HF and GC Buffer Packs

Catalog No. F539L
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Chemical Name or Material:
Detergent-free FF Buffer Pack
Detergent-free GC Buffer Pack
GC Buffer Pack
HF Buffer Pack
Color:
Colorless
Green
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Catalog No. Chemical Name or Material Color
F539L GC Buffer Pack Green
F518L HF Buffer Pack Colorless
F519L GC Buffer Pack Colorless
F520L Detergent-free FF Buffer Pack Colorless
F521L Detergent-free GC Buffer Pack Colorless
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Catalog No. F539L Supplier Thermo Scientific™ Supplier No. F539L
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Description

Includes

Phusion GC Buffer Pack

  • 5X Phusion GC Buffer (1X buffer provides 1.5 mM MgCl2)
  • 50 mM MgCl2
  • DMSO
Phusion Green GC Buffer Pack
  • 5X Phusion Green GC Buffer (1X buffer provides 1.5 mM MgCl2)
  • 50 mM MgCl2
  • DMSO

5X Phusion Green buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel.

The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10-7) is lower than that in GC Buffer (9.5 x 10-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.

5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases:

  • 5X Phusion HF Buffer (F-518L, F-538L (Phusion Green), F-520L (Detergent-free))
  • 5X Phusion GC Buffer (F-519L, F-539L (Phusion Green), F-521L (Detergent-free))

Specifications

Chemical Name or Material GC Buffer Pack
Color Green
For Use With (Application) Standard PCR
Quantity 4 x 1.5 mL
Includes • 5X Phusion Green GC Reaction Buffer (4 x 1.5 mL)
• 50 mM MgCl2 (4 x 1.5 mL)
• DMSO (0.5 mL)

Store at –20°C.

Frequently Asked Questions (FAQs)

Can I use Phusion Green and Phire Green PCR products for subsequent applications such as sequencing, ligation, and restriction digestion?

Yes. The dyes do not interfere with downstream applications such as DNA sequencing, ligation, and restriction digestion.
What dyes are present in Phusion Green and Phire Green buffers?

Phusion Green and Phire Green buffers contain two dyes for monitoring electrophoresis progress. The blue dye migrates with 3-5 kb DNA fragments and the yellow dye migrates faster than 10 bp DNA fragments in 1% agarose gel. The dyes have excitation peaks at 424 nm and 615 nm, respectively.

For Research Use Only. Not for use in diagnostic procedures.