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Molecular Probes™ pHrodo™ Red Zymosan BioParticles™ Conjugate for Phagocytosis

Catalog No. P35364
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Catalog No. P35364 Supplier Molecular Probes™ Supplier No. P35364
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Description

Study endocytosis and phagocytosis regulation by drugs and/or environmental factors with the pHrodo Red Zymosan Bioparticles Conjugate for Phagocytosis, which contains pHrodo Red indicator dye and is compatible with HCS, HTS, fluorescence microplate readers, and flow cytometry.

Achieve faster staining and more accurate results without the need for wash steps or quencher dye with pH-sensitive pHrodo Red Zymosan BioParticles Conjugate for Phagocytosis. This dye-bioparticle conjugate provides faster and more accurate results than any other dye-bioparticle conjugate and has reduced signal variability and improved timing in sensitive experiments. pHrodo Red dye conjugates are non-fluorescent outside the cell, but fluoresce bright red in phagosomes, specifically detecting intracellular phagocytosis and endocytosis. The pHrodo Red Zymosan BioParticles Conjugate can also be multiplexed with a wide variety of blue, green, and far-red dyes and reporters such as GFP, Fuo-4, calcein, NucBlue, CellEvent Caspase 3/7 green, Mitosox Green, and Mitotracker Deep Red, among many others.

The fluorescence of the pHrodo Red dye dramatically increases as pH decreases from neutral to acidic, making it an ideal tool to study phagocytosis and its regulation by drugs and/or environmental factors. Its lack of fluorescence outside the cell eliminates the need for wash steps and quencher dyes.

Use the ready-made pHrodo Red Zymosan BioParticles Conjugate in imaging or flow applications, or pHrodo Red SE, the activated succinimidyl ester, for labeling microorganisms or proteins of your choice.

In addition, pHrodo dye is available in the color green, and with E. coli, zymosan, S. aureus, and dextran 10,000 MW conjugates.

Specifications

Color Red
Content And Storage Store at –20°C, desiccate, and protect from light.
Description pHrodo™ Red Zymosan BioParticles™ Conjugate for Phagocytosis
Detection Method Fluorescence
For Use With (Application) Cell Analysis
For Use With (Equipment) Attune Acoustic Focusing Cytometer, Confocal Microscope, Floid Cell Imaging System, Fluorescence Microscope, High Content Analysis Instrument, Flow Cytometer
Product Type Dye
Dye Type pH-Sensitive Dyes
Product Line pHrodo
Quantity 5 x 1 mg
Shipping Condition Room Temperature
Species S. cerevisiae
Excitation/Emission 560/585
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Frequently Asked Questions (FAQs)

Can I store reconstituted pHrodo BioParticles Conjugates for Phagocytosis and Phagocytosis Kit, for Flow Cytometry?

Yes. Once reconstituted, pHrodo BioParticles Conjugates for Phagocytosis and Phagocytosis Kit, for Flow Cytometry (Cat. Nos. P35367, P35361, P35360, P35366, P35364, P35365, A10010) can be stored at 2 - 8 degrees C for several weeks, as long as sodium azide is added to a final concentration of 2 mM. If no sodium azide is added, the cell suspension needs to be used right away or on the same day to avoid contamination. DO NOT FREEZE the resuspended pHrodo bioparticle conjugates.

Are the Invitrogen BioParticles products sterile?

While the bacteria have been attenuated with formaldehyde and alcohol desiccation, the BioParticles products are not considered sterile, and we do not recommend incubation of more than 4 hours. This applies to all of our dye-labeled (pHrodo, Alexa Fluor, etc.) and unlabeled BioParticles products.
What is the type of bond that attaches the dyes to the BioParticles probes?

We use amine-reactive dyes to covalently attach fluorescent dyes to all of our BioParticles probes such as the Escherichia coli (K-12 strain) BioParticles probes, Staphylococcus aureus (Wood strain without protein A) BioParticles, and the Zymosan A (S. cerevisiae) BioParticles probes.

What can the BioParticles product line be used for?

Fluorescent Bioparticles have been employed to study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry. We offer E.Coli, S. aureus, and zymosan BioParticles conjugates covalently labeled with a variety of different fluorophores.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.