Invitrogen™ Phospho-STAT6 (Tyr641) Monoclonal Antibody (CHI2S4N), PE-Cyanine7, eBioscience™

Mouse Monoclonal Antibody

DescriptionThis CHI2S4N monoclonal antibody recognizes human and mouse signal transducer and activator of transcription 6 (STAT6) when phosphorylated on tyrosine 641. Following their phosphorylation by JAKs, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner. In response to IL-4, STAT6 is phosphorylated on tyrosine 641 by JAK1 and JAK3. STAT6 signaling downstream of the IL-4 receptor promotes T cell growth and B cell production of IgE. Specificity of this CHI2S4N clone was determined by ELISA and flow cytometry.Applications Tested: This CHI2S4N antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generaliz...