Recombinant Protein;50TESTS;PE-Labeled Human PD-1, Fc tag, His tag (recommended for neutralizing assay) (Cat. No. PD1-HP2F2) is produced via conjugation of PE to Human PD-1, Fc tag, His tag under optimal conditions. Human PD-1, Fc tag, His tag is expressed in HEK293 cells containing AA Leu 25 - Gln 167 (Accession # Q15116-1).;This protein carries a human IgG1 Fc tag at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 44.7 kDa. ;Flow Cytometry assay shows that PE-Labeled Human PD-1, Fc Tag, His Tag (recommended for neutralizing assay) (Cat. No. PD1-HP2F2) can bind to 293T cells overexpressing human PD-L1. 1 l stock solution per million cells (QC tested).;FACS analysis shows that the binding of PE-Labeled Human PD-1, Fc Tag, His Tag (recommended for neutralizing assay) (Cat. No. PD1-HP2F2) to 293T overexpressing PD-L1 was inhibited by increasing concentration of neutralizing anti-human PD-L1 antibody. The IC50 is 0.29 g/ml (QC tested).
