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  6. Thermo Scientific™ PageRuler™ Unstained Protein Ladder

Thermo Scientific™ PageRuler™ Unstained Protein Ladder

Numéro de catalogue. pi26614
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2 x 250 μL
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Numéro de catalogue. Quantity
PI26614 2 x 250 μL
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Numéro de catalogue. PI26614 Fournisseur Thermo Scientific™ Code fournisseur 26614
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Description

Unstained mixture of 14 recombinant, highly purified proteins (10kDa to 200kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting.

Thermo Scientific PageRuler Unstained Protein Ladder is a mixture of 14 recombinant, highly purified, unstained proteins (10 to 200 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

Features of the PageRuler Unstained Protein Ladder include:

  • Reference bands—50 kDa band is stronger than the others for easy orientation
  • Tagged—the ladder proteins contains an integral Strep-tag™ II sequence and can be detected on western blots using Strep-Tactin™ conjugates or an antibody against Strep-tag™ II Sequence

Applications

  • Accurate protein sizing on SDS-polyacrylamide gels and western blots

Spécifications

Content And Storage Contents: two vials of 250 μL each, proteins at 0.02 to 0.05 mg/mL of each

Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3, 0.01% bromophenol blue and 33% glycerol

Storage: Upon receipt store at -20°C
Number of Markers 14
Ready to Load Yes
Size Range 10 to 200 kDa
Gel Compatibility Bolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels
Molecular Weight (g/mol) 200, 150, 120, 100, 85, 70, 60, 50, 40, 30, 25, 20, 15, 10 kDa
Quantity 2 x 250 μL
Shipping Condition Approved for shipment on Wet or Dry Ice
Product Line PageRuler
Product Type Protein Ladder
Stain Type Unstained
System Type Western Blotting, SDS-PAGE
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For Research Use Only. Not for use in diagnostic procedures.