Model key characteristics of the adult intestinal epithelium using intestinal organoids established and maintained with this complete medium and optimized protocol. Derive organoids from human intestinal crypts in one week; enable organoid growth across donor samples, including those that are difficult to grow, by an enriched stem cell population. Organoids grown in this medium incorporate a functional lumen enclosed by a polarized intestinal epithelial layer, and can be further differentiated in 3D or in 2D as submerged monolayers or air-liquid interface (ALI) cultures using IntestiCult™ Organoid Differentiation Medium (Human; Catalog #100-0214). Applications of intestinal organoid cultures include developmental studies, disease modelling, investigation of adult stem cell properties, regenerative medicine, and compound screening.Should you intend to use this product for commercial purposes, please contact HUB Organoids B.V. at www.huborganoids.nl
