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Thermo Scientific™ NP-40 Surfact-Amps™ Detergent Solution

Catalog No. PI85125
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Catalog No. PI85125 Supplier Thermo Scientific™ Supplier No. LSG85125
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Description

Thermo Scientific™ Surfact-Amps NP-40 Detergent Solution is highly-purified NP-40 detergent stabilized and supplied as a 10%solution.

Thermo Scientific Surfact-Amps NP-40 Detergent Solution is highly-purified NP-40 detergent stabilized as a 10% solution in your choice of either 10 mL glass ampules or two sizes of HDPE-plastic bottles.

Features of Surfact-Amps NP-40 Detergent Solution:

  • NP-40—a nonionic detergent for use in various protein methods
  • Accurate—precise 10% detergent solutions in ultrapure water
  • Easy-to-use—solutions are simple to dispense and dilute for use
  • Exceptionally pure—less than 1.0μeq/mL peroxides and carbonyls
  • Stable—packaged under inert nitrogen gas in HDPE bottles

This is an easy-to-use 10% (w/v) solution of purified NP-40 detergent for use in routine and high-demand protein research methods and molecular biology techniques. NP-40 is a nonionic polyoxyethylene surfactant that is most frequently used as a component of cell lysis buffers or other solutions intended to extract and solubilize proteins. Surfact-Amps Detergent Solutions (10% w/v) provide unsurpassed purity, quality and stability. Unlike neat detergents, which are extremely viscous, Surfact-Amps 10% Solutions are easy to pipette and accurately dispense. The surfactant solutions are carefully prepared and packaged under nitrogen in glass ampules or non-leaching HDPE bottles, ensuring their stability and eliminating the accumulation of peroxides and degradation products.

Properties of NP-40 Detergent:

  • Molecular Weight: 617 g
  • Detergent Class: Nonionic
  • Aggregation Number: 149
  • Micelle Molecular Weight: 90,000 g
  • Critical Micelle Concentration (CMC): 0.29 mM (0.0179%, w/v)
  • Cloud Point: 80°C
  • Dialyzable: No

Specifications for NP-40 Surfact-Amps Detergent Solution:

  • Visual: Clear, colorless solution, free of particulate matter.
  • Concentration: 10.0±1.0%
  • Oxidants: ≤1.0μeq/mL
  • Carbonyls: ≤1.0μeq/mL
  • Suspended Solids: Residue present must not exceed Residue Reference.

Related Products

  • Surfact-Amps™ Detergent Sampler (Cat. No. 28340)

Specifications

Content And Storage Store between 20°C and 25°C
Form Liquid
Product Line Surfact-Amps
Reagent Type Detergent Solution
Quantity 500 mL
Format HDPE Bottle

Frequently Asked Questions (FAQs)

What are the main advantages of using Thermo Scientific Pierce Surfact-Amps detergents?

Thermo Scientific Pierce Surfact-Amps detergents are highly purified, precisely diluted (10%) formulations that are ideal for applications or assays that are sensitive to contaminants that are present in unpurified detergents. We test every batch to insure that our detergents contain less than 1.0 µeq/mL peroxides and carbonyls and package them under nitrogen, to prevent oxidization during storage.

Superior quality - lower measurable contaminant levels than other leading vendors
Accurate -precise 10% detergent solution in ultrapure water
Easy-to-use - solution is simple to dispense and dilute for use
Exceptionally pure - less than 1.0 µeq/mL peroxides and carbonyls
Are detergents denaturing or non-denaturing with respect to protein structure?

Ionic detergents, or those that carry a charge, are the most likely to be denaturing to proteins. Denaturing detergents can be anionic such as sodium dodecyl sulfate (SDS) or cationic such as ethyl trimethyl ammonium bromide. These detergents totally disrupt membranes and denature proteins by breaking protein-protein interactions through changes in the three-dimensional structure of the proteins. Nondenaturing detergents can be divided into nonionic detergents (i.e., Triton X-100), bile salts (i.e., cholate), and zwitterionic detergents (i.e., CHAPS).
What are detergents?

Detergents are amphipathic molecules containing both a nonpolar “tail” having aliphatic or aromatic character, and a polar “head”. The ionic character of the polar head group forms the basis for broad classification of detergents as ionic, nonionic, or zwitterionic.
How does detergent-based cell lysis work?

Detergents are amphipathic molecules, meaning they contain both a nonpolar “tail” having aliphatic or aromatic character and a polar “head”. Like the components of biological membranes, detergents have hydrophobic-associating properties as a result of their nonpolar tail groups. Nevertheless, detergents are themselves water soluble.

Consequently, detergent molecules allow the dispersion (miscibility) of water-insoluble, hydrophobic compounds into aqueous media, including the extraction and solubilization of membrane proteins. Detergent monomers solubilize membrane proteins by partitioning into the membrane bilayer. With increasing amounts of detergents, membranes undergo various stages of solubilization.
What types of detergents are available for cell lysis?

Detergents can be denaturing or non-denaturing with respect to protein structure. Denaturing detergents can be anionic such as sodium dodecyl sulfate (SDS) or cationic such as ethyl trimethyl ammonium bromide. These detergents totally disrupt membranes and denature proteins by breaking proteinprotein interaction. These detergents are considered harsh. Non-denaturing detergents can be divided into nonionic detergents (i.e., Triton X-100), bile salts (i.e., cholate), and zwitterionic detergents (i.e., CHAPS). These detergents do not denature proteins and do not break protein-protein interactions. These detergents are considered mild.
Why does the method of cell lysis matter?

Cell lysis is the first step in cell fractionation, organelle isolation, and protein extraction and purification. As such, cell lysis opens the door to a myriad of proteomics research methods. Many techniques have been developed and used to obtain the best possible yield and purity for different species of organisms, sample types (cells or tissue), and target molecule or subcellular structure. Subcellular fractionation and protein enrichment are important methods in the rapidly growing field of proteomics. Isolation of subcellular fractions and concentration of proteins in low abundance allow for more efficient identification and study of proteins of interest. Examples are the isolation of integral membrane proteins and nuclear proteins.
What methods of cell lysis are available?

Historically, physical lysis was the method of choice for cell disruption and extraction of cellular contents; however, it often requires expensive, cumbersome equipment and involves protocols that can be difficult to repeat due to variability in the apparatus (such as loose-fitting compared with tight-fitting homogenization pestles). Also, traditional physical disruption methods are not conducive for high-throughput and smaller volumes typical of modern laboratory research.
In recent years, detergent-based cell lysis methods have become the norm. Through empirical testing by trial and error, different detergent-based solutions composed of particular types and concentrations of detergents, buffers, salts and reducing agents have been developed to provide the best possible results for particular species and types of cells. Detergents have both lysing and solubilizing effects.

For Research Use Only. Not for use in diagnostic procedures.