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Invitrogen™ XCell4 SureLock™ Midi-Cell

Catalog No. WR0100
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Catalog No. WR0100 Supplier Invitrogen™ Supplier No. WR0100
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Description

Includes

XCell4 Lower Buffer Chamber; XCell4 Lid with Cables; 2 XCell4 Buffer Cores; XCell4 Buffer Dam; XCell4 Displacement Dam; XCell4 Surelock Assembly; Gel Knife; Set of 2 Power Supply adapters (Zoom Adapters)

Easily set up in just three simple steps

The XCell4 SureLock Midi-Cell allows you to quickly and easily perform simultaneous vertical electrophoresis of 1–4 midi-gels without leaking, resulting in consistent performance. The XCell4 SureLock Midi-Cell is built with tough plastic material to boost durability and features a specially designed lid to enhance user safety.

  • Uses patented technology to make electrophoresis easier and more reliable
  • Designed to dissipate heat effectively and evenly, and ensure high-resolution results when using Novex midi gels (8.7 x 13.3cm)

Flexible, Efficient, and Easy to Use

  • Simple assembly and operation—a single SureLock actuator seals all gels while allowing for zero force insertion and easy positioning of the gels for sample loading
  • Runs up to four Novex midi-gels at once, while having the same approximate footprint as the Criterion™ Cell (Bio-Rad), which runs a maximum of only two gels
  • No need for external cooling accessories–requires 25% less running buffer per gel than the Criterion Cell (340mL vs. 460mL)
How the System Works
  • The XCell4 SureLock Midi-Cell consists of a Lower Buffer Chamber, two Buffer Cores, a lid with cables, a Displacement Dam, and a Buffer Dam
  • Electrophoresis of proteins on midi-gels in the XCell4 SureLock Midi-Cell is performed after assembling each Buffer Core with two midi-gels or a Buffer Core with a midi-gel and a Buffer Dam
  • The Buffer Core/gel assembly is inserted into the Lower Buffer Chamber using a positive locking mechanism that seals the Buffer Core/gel assembly firmly into position and ensures leak-free electrophoresis
  • The Buffer Core/gel assembly creates separate upper buffer chambers for each gel and a common lower buffer chamber shared by all gels
  • The lid is placed onto the Midi-Cell and fits on the Midi-Cell in only one direction
  • The XCell4 SureLock Midi-Cell holds gels firmly with a simple gel tension wedge; when the lever on the gel tension wedge is pushed forward into the locked position, an even horizontal force is generated, which seals the gel/buffer core assembly firmly into position in the lower buffer chamber
  • The positive locking action of the gel tension wedge ensures a trouble-free, leak-free gel run every time

1D Gel Electrophoresis, Protein Gel Electrophoresis, Proteins, Expression, Isolation and Analysis

Order Info

Shipping Condition: Room temperature

Specifications

Content And Storage The components of the midi-cell include:
• 1 XCell4 Lower Buffer Chamber
• 1 XCell4 Lid with Cables
• 2 XCell4 Buffer Cores
• 1 XCell4 Buffer Dam
• 1 XCell4 Displacement Dam
• 1 XCell4 Surelock Assembly
• 1 Gel Knife
• 1 Set of 2 Power Supply adapters (Zoom Adapters).

Operating temperature is 4°C to 40°C. The XCell4 Surelock Midi-Cell Runner is guaranteed for 1 year when properly stored.
For Use With (Equipment) XCell4 SureLock™ Midi
Gel Compatibility Novex™ Midi Gels
Gel Size Midi (8 x 13 cm)
Product Line XCell SureLock
Type Midi-Cell Midi-Gel Electrophoresis System
Quantity 1 Each
Shipping Condition Room Temperature
Max. Voltage 600 VDC

Frequently Asked Questions (FAQs)

Do I need to add water or buffer to the Displacement Dam when I run one or two gels in the XCell4 SureLock Midi-Cell?

The XCell4 Displacement Dam is used when fewer than 3 gels are run in the XCell4 SureLock Midi-Cell. The Displacement Dam is placed in the lower buffer chamber instead of a second buffer core. Do not add any buffer or water to the Displacement Dam. However, if water or buffer is accidentally added to the Displacement Dam, it does not affect the electrophoresis run.
What are the maximum load volumes for the NuPAGE Midi Gels?

Recommended maximum load volumes:
12 + 2-well gel: 45 µL in sample well, 15 µL in marker well
20-well gel: 25 µL
26-well gel: 15 µL
What do I need to run the NuPAGE Invitrogen Midi Gels in the Bio-Rad Criterion Cell?

You will need a Midi Gel Adapter. The Midi Gel Adapter is a self-adhesive, upper buffer chamber used when running the NuPAGE Invitrogen Midi-Gel in a Bio-Rad Criterion Cell. The Midi Gel Adapter may be filled with up to 60 mL of buffer. The Midi Gel Adapters are available packaged with the Midi-Gels (Invitrogen and NuPAGE Invitrogen Midi Gels with Cat. Nos. that end in "A") and also on their own in packages of 10 (Cat. No. WA0999). Running the NuPAGE Midi-Gels in the XCell4 SureLock Gel Apparatus does not require the use of the Midi Gel Adapter.
Are the NuPAGE Midi Gels compatible with multichannel pipettes?

Multichannel pipette compatibility: 12+2-well (8- and 12-channel pipette-compatible) and 26-well (8- and 12-channel pipette-compatible).
The 12-channel pipettes work with the 26-well gels. You will need to alternate lanes (e.g., aligns with every two lanes). There will be two extra lanes (for MW markers). The 12-channel pipettes work with the 12+2 well gels as well.
The leads of the XCell4 SureLock Midi Cell do not fit into my power supply. What should I do?

We recommend using Invitrogen Power Supply Adapters (Cat. No. ZA10001) to help you connect the leads to your power supply. These adapters are supplied along with the XCell4 SureLock Midi-Cell. If these adapters don't work, you may contact the manufacturer of the power supply to see if other options are available.
I am running a Midi gel using the XCell4 SureLock Midi-Cell and the current reading on the power supply is very low. What is the issue?

Here are possible causes and solutions:

- Tape has not been removed from the bottom of the cassette. Remove tape from the bottom of the cassette.
- Connection to power supply not complete. Check all connections with a voltmeter for conductance.
- Insufficient buffer level. Make sure the buffer in the Upper Buffer Chamber (cathode) is covering the wells. Be sure there is sufficient buffer (up to the fill line) in the Lower Buffer Chamber.
- Buffer is leaking from the Upper Buffer Chamber. If the level of running buffer drops, it indicates that the electrophoresis core and cassettes are not properly seated. Repeat the assembly and check to ensure the XCell4 SureLock Assembly is in the locked position.

My Midi gel run in the XCell4 SureLock Midi-Cell is taking longer than usual. Can you please offer some tips?

Here are possible causes and solutions:

- Voltage is set too low. Set correct voltage as described in the manual.
- Buffers are too dilute. Check buffer recipe; re-make if necessary.
- Upper buffer chamber is leaking. Make sure the buffer core is firmly seated. If you are using the buffer dam, make sure the dam is properly positioned in the core. If the core gasket is damaged, replace with a fresh gasket. Check to ensure that the XCell4 SureLock Assembly is in the locked position.

How do you recommend cleaning the XCell4 SureLock Midi-Cell?

We recommend washing the unit with a mild detergent and rinsing with deionized water after each use.
What are the power supply requirements for the XCell4 SureLock Midi-Cell? Do you offer a power supply that I can use with it?

The XCell4 SureLock Midi-Cell should be used with an external DC power supply designed for electrophoresis. This power supply must:

- Be isolated from the ground so that the DC output is floating (safety requirement)and must be equipped with No Load or Open Circuit protection
- Be able to operate at 200 V

If you would like to simultaneously run 4 gels using the XCell4 SureLock Midi-Cell, we recommend using the ZOOM Dual Power Supply. The Low Voltage/High Current outlets can handle the voltage and current of 4 NuPAGE Bis-Tris gels (Voltage 200 V, Current ~800 mA). The PowerEase 90W Power Supply and PowerEase 500 Power Supply (discontinued as of December 31, 2013) can be used with the XCell4 SureLock Midi-Cell but the current limit of 500 mA will be reached with 2 gels (2 x ~200 mA max current at 200V). If 4 gels are run in the XCell4 SureLock Midi-Cell with these power supplies, the current will be maxed out and the run will slow down. It will take approximately twice as long to run the gels. Note: To allow connection of the covered power leads of the XCell4 SureLock Midi-Cell with certain power supplies that are not designed for use with covered or non-retractable power leads, use Invitrogen Power Supply Adapters available separately (Cat. No. ZA10001).

Can I use the gel tension wedge that is supplied with the XCell SureLock Mini-Cell for the XCell4 SureLock Midi-Cell?

We do not recommend using the gel tension wedge that is supplied with the XCell SureLock Mini-Cell for the XCell4 SureLock Midi-Cell as it is specifically designed for the Mini-Cell and will not fit correctly in the Midi-Cell.
What is the main purpose of the XCell4 SureLock Assembly?

The XCell4 SureLock Assembly or CAM is similar to a gel tension wedge. The positive locking action of the XCell SureLock Assembly ensures trouble-free, leak-free electrophoresis of protein gels. Move the lever on the XCell SureLock Assembly to a locked position to generate an even horizontal force which seals the gel cassette/Buffer Core assembly firmly into position in the Lower Buffer Chamber. The locked and unlocked positions are marked on the XCell SureLock Assembly for easy, intuitive assembly.
When should I use the XCell4 Displacement Dam with the XCell4 SureLock Midi-Cell?

Use the XCell4 Displacement Dam when you wish to run less than 3 gels in the XCell4 SureLock Midi-Cell. The Displacement Dam is placed in the Lower Buffer Chamber instead of a second Buffer Core. Do not add any buffer or water to the Displacement Dam. However, if water or buffer is accidentally added to the Displacement Dam, it does not affect the electrophoresis run.
What is the tolerance of the XCell4 SureLock Midi-Cell to organic solvents?

The XCell4 SureLock Midi-Cell is impervious to alcohol, but not compatible with chlorinated hydrocarbons (e.g., chloroform), aromatic hydrocarbons (e.g., toluene, benzene) or acetone.
Can I purchase replacement parts for the XCell4 SureLock Midi-Cell?

Here are the replacement parts we offer for the XCell4 SureLock Midi-Cell. They can only be ordered by phone:

- XCell4 SureLock Midi-Cell Lid: Cat. No. WR0101
- XCell4 SureLock Midi-Cell Lower Buffer Chamber: Cat. No. WR0102
- XCell4 SureLock Midi-Cell Displacement Dam: Cat. No. WR0106
- XCell SureLock Upgrade kit: Cat. No. WR0112 (contains 1 SureLock Assembly, 1 buffer dam, and 2 cores) Please note that in addition to ordering on the phone by calling Customer Service, this item can also be ordered using the “Quick Order” link (https://www.thermofisher.com/store/quick-order) that can be found at the top right of most pages on our website. The price will display when the item is added to your shopping cart.
Can I run my Bio-Rad Criterion gels using the XCell4 SureLock Midi-Cell?

No. While the NuPAGE Invitrogen Midi Gels are compatible with the Bio-Rad Criterion system (when the specific adapter is used), the Bio-Rad Criterion gels will not work with the XCell4 SureLock Midi-Cell. Note: Our Mini gels are not compatible with the Bio-Rad Mini-PROTEAN apparatus.

Can I run 1 gel using the XCell4 SureLock Midi-Cell?

Yes, the XCell4 SureLock Midi-Cell is designed to perform simultaneous vertical electrophoresis of 1-4 midi-gels. Use the XCell4 Displacement Dam when you wish to run less than 3 gels in the XCell4 SureLock Midi-Cell. The Displacement Dam is placed in the Lower Buffer Chamber instead of a second Buffer Core. Do not add any buffer or water to the Displacement Dam. However, if water or buffer is accidentally added to the Displacement Dam, it does not affect the electrophoresis run.
Which of your protein gels can I run using the XCell4 SureLock Midi-Cell?

Our Midi gels can be run using the XCell4 SureLock Midi-Cell.
What are the components of the XCell4 SureLock Midi-Cell?

The XCell4 SureLock Midi-Cell includes the lower buffer chamber, lid with cables, two buffer cores, buffer dam, displacement dam, SureLock assembly, and gel knife. For descriptions and images of the various parts, please refer to pages 1-3 of the product manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/xcell4_midicell_man.pdf).

For Research Use Only. Not for use in diagnostic procedures.