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Invitrogen™ Ni-NTA Agarose

Catalog No. R90115
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R90101 10 mL
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Catalog No. R90115 Supplier Invitrogen™ Supplier No. R90115
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Description

Includes

The Ni-NTA resin is pre-charged and able to bind up to 50mg of recombinant protein per 1mL of resin. It is provided as a 50% slurry in 30% ethanol. The resin will appear blue in color when charged with Ni2+

Nickel-charged affinity resin that can be used to purify recombinant proteins containing a polyhistidine (6xHis) sequence

  • Ni-NTA Agarose is a nickel-charged affinity resin that can be used to purify recombinant proteins containing a polyhistidine (6xHis) sequence
  • Proteins bound to the resin may be eluted with either low pH buffer or by competition with imidazole or histidine
  • One-step purification can be performed under both native and denaturing conditions
  • Ni-NTA uses the chelating ligand nitrilotriacetic acid (NTA) coupled to a cross-linked 6% agarose resin that is suitable for use in batch and gravity flow applications
  • Guaranteed stable for 6 months when properly stored at 4°C

Specifications

Content And Storage The Ni-NTA resin is pre-charged and able to bind up to 50 mg of recombinant protein per 1 ml of resin. It is provided as a 50% slurry in 30% ethanol. The resin will appear blue in color when charged with Ni2+ . Store at +4°C. Ni-NTA resin is guaranteed stable for 6 months when properly stored.
Stationary Phase Ni-NTA
Type Agarose
Quantity 25 mL
Form Suspension
Column Type Affinity Column

Frequently Asked Questions (FAQs)

Can ProBond or Ni-NTA beads be used for large-scale preparations?

ProBond and Ni-NTA beads can be used in FPLC columns. However, the beads can only withstand low pressure (~43.5 psi max).
What should the typical protein recovery be when using the Probond Purification System or Ni-NTA Purification system?

Both systems are qualified by purifying 2 mg of myoglobin protein on a column and performing a Bradford assay. Protein recovery must be 75% or higher.

For Research Use Only. Not for use in diagnostic procedures.