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Invitrogen™ Midi-Gel Adapters

Catalog No. WA0999
Encompass
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WA0999 Pack of 10
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Catalog No. WA0999 Supplier Invitrogen™ Supplier No. WA0999
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Description

Includes

One box of 10 gels

Designed for use with the Novex Midi Gel Cassette to perform electrophoresis

 

  • Supports use of BioRad Criterion Cell
  • Also supplied with the Novex Midi Gels with Adapters Kit
  • Includes one pack of 10 gels.

 

1D Gel Electrophoresis, Protein Gel Electrophoresis, Proteins, Expression, Isolation and Analysis

Order Info

Shipping Condition: Room Temperature

Specifications

Content And Storage 10/pack
Gel Size Midi
For Use With (Application) Protein Electrophoresis
Gel Compatibility Novex™ Midi Gel Cassette (NuPAGE™ or Tris-Glycine Gels)
Quantity Pack of 10
Shipping Condition Room Temperature
Product Line Novex
Product Type Midi Gel Adapter
For Use With (Equipment) Midi Gel Tank

Frequently Asked Questions (FAQs)

Do I need to add water or buffer to the Displacement Dam when I run one or two gels in the XCell4 SureLock Midi-Cell?

The XCell4 Displacement Dam is used when fewer than 3 gels are run in the XCell4 SureLock Midi-Cell. The Displacement Dam is placed in the lower buffer chamber instead of a second buffer core. Do not add any buffer or water to the Displacement Dam. However, if water or buffer is accidentally added to the Displacement Dam, it does not affect the electrophoresis run.
What are the maximum load volumes for the NuPAGE Midi Gels?

Recommended maximum load volumes:
12 + 2-well gel: 45 µL in sample well, 15 µL in marker well
20-well gel: 25 µL
26-well gel: 15 µL
What do I need to run the NuPAGE Invitrogen Midi Gels in the Bio-Rad Criterion Cell?

You will need a Midi Gel Adapter. The Midi Gel Adapter is a self-adhesive, upper buffer chamber used when running the NuPAGE Invitrogen Midi-Gel in a Bio-Rad Criterion Cell. The Midi Gel Adapter may be filled with up to 60 mL of buffer. The Midi Gel Adapters are available packaged with the Midi-Gels (Invitrogen and NuPAGE Invitrogen Midi Gels with Cat. Nos. that end in "A") and also on their own in packages of 10 (Cat. No. WA0999). Running the NuPAGE Midi-Gels in the XCell4 SureLock Gel Apparatus does not require the use of the Midi Gel Adapter.
Are the NuPAGE Midi Gels compatible with multichannel pipettes?

Multichannel pipette compatibility: 12+2-well (8- and 12-channel pipette-compatible) and 26-well (8- and 12-channel pipette-compatible).
The 12-channel pipettes work with the 26-well gels. You will need to alternate lanes (e.g., aligns with every two lanes). There will be two extra lanes (for MW markers). The 12-channel pipettes work with the 12+2 well gels as well.
How much of the pAd/CMV/V5-DEST or pAd/PL-DESTpAD destination vector do I need to add in the LR reaction?

One should use 2 µl = 300 ng pAd-DEST vector. Please Note that Version D of the pAd/CMV/V5-DEST and pAd/PL-DEST Gateway Vectors product manual (manual number 25-0544) had an error on page 9 regarding this protocol. The manual instructed users to add 1 µl, but this has since been corrected to reflect the correct protocol of addition of 2 µl.

For Research Use Only. Not for use in diagnostic procedures.